Antiviral effect of silymarin against Zika virus in vitro.

Simple item page
dc.contributor.authorSilva, Tales Fernando da
dc.contributor.authorFerraz, Ariane Coelho
dc.contributor.authorAlmeida, Letícia Trindade
dc.contributor.authorCaetano, Camila Carla da Silva
dc.contributor.authorCamini, Fernanda Caetano
dc.contributor.authorLima, Rafaela Lameira Souza
dc.contributor.authorAndrade, Ana Cláudia dos Santos Pereira
dc.contributor.authorOliveira, Danilo Bretas de
dc.contributor.authorRocha, Kamila Lorene Soares
dc.contributor.authorSilva, Breno de Mello
dc.contributor.authorMagalhães, José Carlos de
dc.contributor.authorMagalhães, Cíntia Lopes de Brito
dc.date.accessioned2021-09-27T15:11:03Z
dc.date.available2021-09-27T15:11:03Z
dc.date.issued2020pt_BR
dc.description.abstractZika virus (ZIKV) epidemic and its association with severe neurological syndromes have raised worldwide concern. Despite the great clinical relevance of this infection, no vaccine or specific treatment is available and the search for antiviral compounds against ZIKV is extremely necessary. Several natural compounds, such as silymarin, exhibit antioxidant, hepatoprotective, and antiviral properties; however, the antiviral potential of this compound remains partially investigated. Therefore, the objective of this study was to evaluate in vitro the antiviral activity of silymarin against ZIKV infection. Global antiviral activity, dose-dependent, plaque reduction, and time-of-drug-addition assays were used to determine the anti-ZIKV activity of silymarin. Additionally, to start characterizing the mechanisms of action we determined whether silymarin could have a virucidal effect and inhibit viral adsorption and penetration stages. Regarding its global antiviral activity, silymarin showed significant inhibition of ZIKV infection, protecting cells infected with EC50 equal to 34.17μg/mL, with a selectivity index greater than 17 and 4x greater than that of the positive control (ribavirin). Its greatest efficiency was achieved at 125μg/mL, whose cell viability did not differ from the control without infection and treatment. Furthermore, treatment with silymarin reduced viral load by up to two logs (> 90%) concerning viral control, when evaluating virucidal activity and the precocious times of infection. Thus, our results set to show the promising anti-ZIKV activity of silymarin, which does not seem to have a single inhibition mechanism, acting at different times of infection, and still has the advantage of silymarin be a phytotherapy already available on the market.pt_BR
dc.identifier.citationSILVA, T. F. da et al. Antiviral effect of silymarin against Zika virus in vitro. Acta Tropica, v. 211, artigo 105613, nov. 2020. Disponível em: <https://www.sciencedirect.com/science/article/abs/pii/S0001706X2030824X?via%3Dihub>. Acesso em: 10 jun. 2021.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.actatropica.2020.105613pt_BR
dc.identifier.issn0001-706X
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/13811
dc.identifier.uri2https://www.sciencedirect.com/science/article/abs/pii/S0001706X2030824X?via%3Dihubpt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.titleAntiviral effect of silymarin against Zika virus in vitro.pt_BR
dc.typeArtigo publicado em periodicopt_BR
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ARTIGO_AntiviralEffectSilymarin.pdf
Size:
645.02 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
DECBI - Artigos publicados em periódicos