Identification of new genes related to virulence of xanthomonas axonopodis pv. citri during citrus host interactions.
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Date
2017
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Abstract
A mutant library of the bacterium Xanthomonas citri subsp. citri strain 306 pathotype
A (Xac ), the causative agent of most aggressive Asiatic type A citrus canker, was
screened regarding altered canker symptoms after inoculations into Citrus sinensis
and Citrus limonia host leaves. Twenty-six mutants have shown phenotypic virulence
changes and have respectively knocked out gene identified by sequencing. In
vivo growth curves were obtained for nine mutants to quantify how the mutations
could affect pathogen’s adaptability to growth inside and attack host plant infected
tissue. Among identified genes in mutated strains, we could find those that until now
had not been reported as being involved in Xac adaptation and/or virulence, such as
predicted to encode for xylose repressor-like protein (XACΔxylR), Fe-S oxidoredutase
(XACΔaslB), helicase IV (XACΔhelD), ubiquinol cytochrome c oxidoreductase
iron-sulfur subunit (XACΔpetA), chromosome partitioning protein (XACΔparB)
and cell division protein FtsB (XACΔftsB), in addition to genes predicted to encode
for hypothetical proteins. The new genes found in this study as being relevant to
adaptation and virulence, improve the understanding of Xac fitness during citrus
plant attack and canker symptoms development.
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Keywords
Mutants, Impaired Virulence
Citation
FERREIRA, C. B. et al. Identification of new genes related to virulence of xanthomonas axonopodis pv. citri during citrus host interactions. Advances in Microbiology, v. 07, p. 22-46, 2017. Disponível em: <http://www.scirp.org/journal/PaperInformation.aspx?paperID=73334> Acesso em: 15 set. 2017.