Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis.

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dc.contributor.authorFerreira, Rafael Marini
dc.contributor.authorMoreira, Leandro Marcio
dc.contributor.authorFerro, Jesus Aparecido
dc.contributor.authorSilva, Marcia Regina Soares da
dc.contributor.authorLaia, Marcelo Luiz de
dc.contributor.authorVarani, Alessandro de Mello
dc.contributor.authorOliveira, Julio Cezar Franco de
dc.contributor.authorFerro, Maria Inês Tiraboschi
dc.date.accessioned2016-07-25T16:21:33Z
dc.date.available2016-07-25T16:21:33Z
dc.date.issued2016
dc.description.abstractCitrus canker is a major disease affecting citrus production in Brazil. It's mainly caused by Xanthomonas citri subsp. citri strain 306 pathotype A (Xac). We analysed the differential expression of proteins secreted by wild type Xac and an asymptomatic mutant for hrpB4 (1hrpB4) grown in Nutrient Broth (NB) and a medium mimicking growth conditions in the plant (XAM1). This allowed the identification of 55 secreted proteins, of which 37 were secreted by both strains when cultured in XAM1. In this secreted protein repertoire, the following stand out: Virk, Polyphosphate-selective porin, Cellulase, Endoglucanase, Histone-like protein, Ribosomal proteins, five hypothetical proteins expressed only in the wild type strain, Lytic murein transglycosylase, Lipoprotein, Leucyl-tRNA synthetase, Co-chaperonin, Toluene tolerance, C-type cytochrome biogenesis membrane protein, Aminopeptidase and two hypothetical proteins expressed only in the1hrpB4 mutant. Furthermore, Peptidoglycan-associated outer membrane protein, Regulator of pathogenicity factor, Outer membrane proteins, Endopolygalacturonase, Chorismate mutase, Peptidyl-prolyl cis-trans isomerase and seven hypothetical proteins were detected in both strains, suggesting that there was no relationship with the secretion mediated by the type III secretory system, which is not functional in the mutant strain. Also worth mentioning is the Elongation factor Tu (EF-Tu), expressed only the wild type strain, and Type IV pilus assembly protein, Flagellin (FliC) and Flagellar hook-associated protein, identified in the wild-type strain secretome when grown only in NB. Noteworthy, that FliC, EF-Tu are classically characterized as PAMPs (Pathogen-associated molecular patterns), responsible for a PAMP-triggered immunity response. Therefore, our results highlight proteins potentially involved with the virulence. Overall, we conclude that the use of secretome data is a valuable approach that may bring more knowledge of the biology of this important plant pathogen, which ultimately can lead to the establishment of new strategies to combat citrus canker.pt_BR
dc.identifier.citationFERREIRA, F. M. et al. Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis. PEERJ, v. 4, p. e1734, 2016. Disponível em: <https://peerj.com/articles/1734/#additional-information>. Acesso em: 16 jun. 2016.pt_BR
dc.identifier.doihttps://doi.org/10.7717/peerj.1734/supp-1
dc.identifier.issn2167-9843 
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/6629
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseDistributed under Creative Commons CC-BY 4.0 which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fonte: o próprio artigo.pt_BR
dc.subjectPlant pathogen interactionpt_BR
dc.subjectMedium inducing pathogenicitypt_BR
dc.subjectType III secretion systempt_BR
dc.subjectAsymptomatic mutantpt_BR
dc.subjectVirulencept_BR
dc.titleUnravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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