Silva, Thais Almeida Marques daGomes, Luciana InáciaOliveira, EdwardVital, Wendel CouraSilva, Letícia de AzevedoPais, Fabiano Sviatopolk MirskyKer, Henrique GamaReis, Alexandre BarbosaRabello, Ana Lúcia TelesCarneiro, Mariângela2016-01-212016-01-212015SILVA, T. A. M. da et al. Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA), Minas Gerais, Brazil. Parasites & Vectors, v. 8, p. 226e, 2015. Disponível em: <http://www.parasitesandvectors.com/content/8/1/226>. Acesso em: 15 out. 2015.1756-3305http://www.repositorio.ufop.br/handle/123456789/6195Background: Certain municipalities in the Belo Horizonte Metropolitan Area (BHMA), Minas Gerais, Brazil, have the highest human visceral leishmaniasis (VL) mortality rates in the country and also demonstrate high canine seropositivity. In Brazil, the etiologic agent of VL is Leishmania (Leishmania) infantum. The aim of this study was to evaluate the intraspecific genetic variability of parasites from humans and from dogs with different clinical forms of VL in five municipalities of BHMA using PCR-RFLP and two target genes: kinetoplast DNA (kDNA) and gp63. Methods: In total, 45 samples of DNA extracted from clinical samples (n = 35) or L. infantum culture (n = 10) were evaluated. These samples originated from three groups: adults (with or without Leishmania/HIV co-infection; n = 14), children (n = 18) and dogs (n = 13). The samples were amplified for the kDNA target using the MC1 and MC2 primers (447 bp), while the Sg1 and Sg2 (1330 bp) primers were used for the gp63 glycoprotein target gene. Results: The restriction enzyme patterns of all the samples tested were monomorphic. Conclusions: These findings reveal a high degree of genetic homogeneity for the evaluated gene targets among L. infantum samples isolated from different hosts and representing different clinical forms of VL in the municipalities of BHMA studied.en-USArtigo publicado em periodicoThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. Fonte: o próprio artigo.https://doi.org/10.1186/s13071-015-0837-y