Rodrigues, Patrísia de OliveiraBarreto, Elisa da SilvaBrandão, Rogélio LopesGurgel, Leandro Vinícius AlvesPasquini, DanielBaffi, Milla Alves2022-12-072022-12-072022RODRIGUES, P. de O. et al. On‐site produced enzyme cocktails for saccharifcation and ethanol production from sugarcane bagasse fractionated by hydrothermal and alkaline pretreatments. Waste and Biomass Valorization, v. 13, p. 95-106, 2022. Disponível em: <https://link.springer.com/article/10.1007/s12649-021-01499-7>. Acesso em: 11 out. 2022.1877-265Xhttp://www.repositorio.ufop.br/jspui/handle/123456789/15868Enzymatic blends produced by fungal monocultures and consortia cultured in solid-state fermentation (SSF), using sugarcane bagasse (SB) and wheat bran as substrates (1:1, w/w), were evaluated for saccharifcation of sugarcane bagasse pretreated by autohydrolysis (hydrothermal pretreatment—HP) and alkaline delignifcation (HP-Soda). The highest glucose releases were obtained after saccharifcations of SB pretreated by HP using enzyme cocktails produced by Aspergillus niger and by the consortium among A. fumigatus, Ganoderma lucidum and Trametes versicolor, with 10.8 and 9.8 g L−1, respectively. For SB pretreated by HP-Soda, the hydrolysate 10 (extract from A. niger, G. lucidum and Pleurotus ostreatus consortium) achieved maximal glucose concentration (11.92 g L−1). After alcoholic fermentation of the hydrolysates, the greatest ethanol yield in relation to the maximum theoretical yield (60.8%) was obtained in the fermentation of hydrolysate 1 (A. niger) obtained from SB pretreated by HP-Soda. These results demonstrated that on-site produced enzyme cocktails can be applied for sac- charifcation of pretreated sugarcane bagasse and also contribute to cost reduction of bioconversion processes.en-USrestritoAutohydrolysisSoda pretreatmentArtigo publicado em periodicohttps://link.springer.com/article/10.1007/s12649-021-01499-7https://doi.org/10.1007/s12649-021-01499-7