Oliveira, Karla Andrade deOliveira, Luciana Souza deDias, C. C. A.Silva Júnior, AbelardoLamëgo, Márcia Rogéria de AlmeidaAmalda, G.Bouyer, Donald H.Galvão, Márcio Antônio MoreiraSiqueira, Cláudio Lísias Mafra de2014-11-112014-11-112008OLIVEIRA, K. A. de et al. Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. Memórias do Instituto Oswaldo Cruz, v. 103, p. 191-194, 2008. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200011&lng=en&nrm=iso&tlng=en>. Acesso em: 03 set. 2014.0074-0276http://www.repositorio.ufop.br/handle/123456789/3785Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa pro¬tein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previ¬ously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.en-USRickettsia felisRickettsia rickettsiiCtenocephalides felisBrazilian spotted feverTickArtigo publicado em periodicoO periódico Memórias do Instituto Oswaldo Cruz permite que o Repositório Institucional da Universidade Federal de Ouro Preto (UFOP) deposite uma cópia eletrônica dos artigos publicados por esse periódico em que ao menos um dos autores faça parte da comunidade cientifica da UFOP. Fonte: Licença concedida mediante prenchimento de formulário enviado no dia 12/12/2013.http://dx.doi.org/10.1590/S0074-02762008000200011