Ribeiro, Iara Mariana LéllisPereira, Wagner LuizNogueira, Leonardo BrandãoOliveira, Laser Antônio Machado deTeixeira, Róbson RicardoNogueira, Katiane de Oliveira Pinto Coelho2021-11-222021-11-222020RIBEIRO, I. M. L. et al. Neuroprotective effect of isobenzofuranones on hydrogen peroxide-mediated redox imbalance in primary cultures of hippocampal neurons. Brazilian Archives of Biology and Technology, v. 63, p. e20190072, 2020. Disponível em: <https://www.scielo.br/j/babt/a/Gwz5DJnmwjGwQJ7kWKtc5GH/abstract/?lang=en>. Acesso em: 10 jun. 2021.1678-4324http://www.repositorio.ufop.br/jspui/handle/123456789/13987In live organisms, there is a balance between the production of reactive oxygen species (ROS) and their neutralization. The increased level of these species leads to a condition called redox imbalance. The aim of this study was to evaluate the protective action of isobenzofuranones in primary cultures of hippocampal neurons subjected to redox imbalance. To accomplish this, MTT and LIVE/DEAD assays were initially performed. In the cultures pretreated with isobenzofuranones 1 and 2, there was a higher number of live cells when compared to that in the untreated ones. Regarding redox imbalance, there was a significant increase in the intracellular levels of ROS. The cultures pretreated with isobenzofuranones showed a reduction in ROS levels. Lipid peroxidation caused by oxidative damage was significantly reduced in the cultures pretreated with isobenzofuranones 1 and 2. Taken together, these data show the ability of isobenzofuranones 1 and 2 to significantly minimize cytotoxicity, cell death, intracellular levels of ROS and lipid peroxidation induced by redox imbalance. These results suggest that isobenzofuranones 1 and 2 represent a possible alternative therapy for the neurodegenerative disturbances that are triggered by ROS production increases.en-USabertoReactive oxygen speciesPhthalideArtigo publicado em periodicoSubmitted for possible open access publication under the terms and conditions of the Creative Commons Attribution (CC BY NC) license (https://creativecommons.org/licenses/by-nc/4.0/). Source: The article PDF.https://doi.org/10.1590/1678-4324-2020190072