Maia, Ana Carolina Ribeiro GomesPorcino, Gabriane NascimentoDetoni, Michelle de LimaEmídio, Nayara BragaMarconato, Danielle GomesPinto, Priscila de FariaFessel, Melissa ReginaReis, Alexandre BarbosaJuliano Neto, LuizJuliano, Maria AparecidaMarques, Marcos JoséVasconcelos, Eveline Gomes2017-02-152017-02-152013MAIA, A. C. R. G. et al. An antigenic domain within a catalytically active leishmania infantum nucleoside triphosphate diphosphohydrolase (NTPDase 1) is a target of inhibitory antibodies. Parasitology International, v. 18, p.44-52, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1383576912001274>. Acesso em: 10 out. 2016.1383-5769http://www.repositorio.ufop.br/handle/123456789/7263Weidentified a shared B domainwithin nucleoside triphosphate diphosphohydrolases (NTPDases) of plants and parasites. Now, an NTPDase activity not affected by inhibitors of adenylate kinase and ATPases was detected in Leishmania infantum promastigotes. By non-denaturing gel electrophoresis of detergent-homogenized promastigote preparation, an active band hydrolyzing nucleosides di- and triphosphate was visualized and, following SDS-PAGE and silver staining was identified as a single polypeptide of 50 kDa. By Western blots, it was recognized by immune sera raised against potato apyrase (SA), r-pot B domain (SB), a recombinant polypeptide derived fromthe potato apyrase, and LbB1LJ (SC) or LbB2LJ (SD), synthetic peptides derived fromthe Leishmania NTPDase 1, and by serum samples from dogs with visceral leishmaniasis, identifying the antigenic L. infantum NTPDase 1 and, also, its conserved B domain (r83–122). By immunoprecipitation assays andWestern blots, immune sera SA and SB identified the catalytically active NTPDase 1 in promastigote preparation. In addition, the immune sera SB (44%) and SC or SD (87–99%) inhibited its activity, suggesting a direct effect on the B domain. By ELISA, 37%, 45% or 50% of 38 infected dogs were seropositive for r-pot B domain, LbB1LJ and LbB2LJ, respectively, confirming the B domain antigenicity.en-USrestritoPotato apyraseConserved domainVisceral leishmaniasisArtigo publicado em periodicohttp://www.sciencedirect.com/science/article/pii/S1383576912001274https://doi.org/10.1016/j.parint.2012.09.004