Côrtes, Denise FonsecaCarneiro, Matheus Batista HeitorSantos, Liliane Martins dosSouza, Talita Correia de OliveiraMaioli, Tatiani UceliDuz, Ana Luiza CassinJorge, Maria Letícia RamosAfonso, Luís Carlos CroccoCarneiro, Cláudia MartinsVieira, Leda Quercia2015-11-262015-11-262010CÔRTES, D. F. et al. Low and high-dose intradermal infection with Leishmania major and Leishmania amazonensis in C57BL/6 mice. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 105, n. 6, p. 736-745, set. 2010. Disponível em: <http://www.scielo.br/pdf/mioc/v105n6/02.pdf>. Acesso em: 21 out. 20151678-8060http://www.repositorio.ufop.br/handle/123456789/5834A model of skin infection with Leishmania amazonensis with low doses of parasites is compared to infection with high doses of L. amazonensis and low and high doses of Leishmania major. C57BL/6 mice were infected with 103 or 106 parasites in the ear and the outcome of infection was assessed. The appearance of lesions in mice infected with 103 parasites was delayed compared to mice infected with 106 Leishmania and parasites were detectable at the infection site before lesions became apparent. Mice infected with L. amazonensis displayed persistent lesions, whereas infection with L. major spontaneously healed in all groups, although lymphocytes persisted at the site of infection after healing. Macrophages persisted only in L. amazonensis-infected mice. High-dose L. amazonensis-infected mice produced lower levels of IFN-γ and TNF than mice infected with L. major. No correlation between the persistence of parasites and IL-10 levels and the production of nitric oxide or urea by macrophages was found. We conclude that infection with low doses of L. amazonensis in the dermis changes the course of infection by delaying the appearance of lesions. However, low-dose infection does not change the outcomes of susceptibility and cytokine production described for subcutaneous infection with high numbers of parasites.en-USCytokinesArtigo publicado em periodicoTodo o conteúdo do periódico Memórias do Instituto Oswaldo Cruz, exceto onde identificado, está sob uma licença Creative Commons que permite copiar, distribuir e transmitir o trabalho em qualquer suporte ou formato desde que sejam citados o autor e o licenciante. Não permite o uso para fins comerciais. Fonte: Memórias do Instituto Oswaldo Cruz <http://www.scielo.br/scielo.php/script_sci_serial/pid_0074-0276/lng_pt/nrm_iso>. Acesso em: 19 ago. 2019.http://dx.doi.org/10.1590/S0074-02762010000600002