Resende, Daniela de MeloCaetano, Bráulia CostaDutra, Míriam SantosAbrantes, Christiane de FreitasVerly, Rodrigo MoreiraResende, Jarbas MagalhãesVeloso, Dorila PilóRezende, Simone AparecidaRomero, Oscar BrunaFernandes, Ana PaulaGazzinelli, Ricardo Tostes2014-11-202014-11-202008RESENDE, D. de M. et al. Epitope mapping and protective immunity elicited by adenovirus expressing the Leishmania amastigote specific A2 antigen : correlation with IFN- and cytolytic activity by CD8+ T cells. Vaccine, v. 26, p. 4585-4593, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0264410X08006932>. Acesso em: 28 ago. 2014.0264-410Xhttp://www.repositorio.ufop.br/handle/123456789/4003A2was identified as an amastigote virulence factor of Leishmania (Leishmania) donovani and as a candidate antigen for vaccine development against visceral leishmaniasis. Here, predicted hydrophilic, class I and II MHC-binding synthetic peptides were used to define epitopes recognized by A2-specific antibodies, CD8+ T and CD4+ T cells, respectively. Immunization of BALB/c mice with adenovirus expressing A2 (AdA2) resulted in lowantibody response, contrasting with high levels of IFN-_ producing CD4+ T and CD8+ T cells specific for A2. Further, A2-specific CD8+ T cells from immunized mice were capable of lysing sensitized target cells in vivo. Finally, we demonstrated an association of A2-specific T cell responses and reduced parasitism in both liver and spleen from mice immunized with AdA2 and challenged with L. (L.) chagasi.en-USLeishmaniasisRecombinant vaccineEpitope mappingArtigo publicado em periodicoO periódico Vaccine concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3460890061017.https://doi.org/10.1016/j.vaccine.2008.05.091