Dornas, Waleska Claudia AmaralLima, Wanderson Geraldo deSantos, Rinaldo Cardoso dosGuerra, Joyce Ferreira da CostaSouza, Melina Oliveira deSilva, MaísaSilva, Lorena Souza eDiniz, Mirla FiuzaSilva, Marcelo Eustáquio2015-03-122015-03-122013DORNAS, W. C. et al. High dietary salt decreases antioxidant defenses in the liver of fructose-fed insulin-resistant rats. Journal of Nutritional Biochemistry, v. 1, p. 1, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0955286313001381>. Acesso em: 08 nov. 2014.0955-2863http://www.repositorio.ufop.br/handle/123456789/4602In this study we investigated the hypothesis that a high-salt diet to hyperinsulinemic rats might impair antioxidant defense owing to its involvement in the activation of sodium reabsorption to lead to higher oxidative stress. Rats were fed a standard (CON), a high-salt (HS), or a high-fructose (HF) diet for 10 weeks after which, 50% of the animals belonging to the HF group were switched to a regimen of high-fructose and high-salt diet (HFS) for 10 more weeks, while the other groups were fed with their respective diets. Animals were then euthanized and their blood and liver were examined. Fasting plasma glucose was found to be significantly higher (approximately 50%) in fructose-fed rats than in the control and HS rats, whereas fat liver also differed in these animals, producing steatosis. Feeding fructose-fed rats with the high-salt diet triggered hyperinsulinemia and lowered insulin sensitivity, which led to increased levels of serum sodium compared to the HS group. This resulted in membrane perturbation, which in the presence of steatosis potentially enhanced hepatic lipid peroxidation, thereby decreasing the level of antioxidant defenses, as shown by GSH/GSSG ratio (HFS rats, 7.098±2.1 versus CON rats, 13.2±6.1) and superoxide dismutase (HFS rats, 2.1±0.05 versus CON rats, 2.3±0.1%), and catalase (HFS rats, 526.6±88.6 versus CON rats, 745.8±228.7 U/mg ptn) activities. Our results indicate that consumption of a salt-rich diet by insulin-resistant rats may lead to regulation of sodium reabsorption, worsening hepatic lipid peroxidation associated with impaired antioxidant defenses.en-USHigh salt dietFructose fed ratsOxidative stressSteatosisAntioxidant defensesArtigo publicado em periodicoO periódico The Journal of Nutritional Biochemistry concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3550930672562.https://doi.org/10.1016/j.jnutbio.2013.06.006