Guedes, Paulo Marcos da MattaVeloso, Vanja MariaAfonso, Luís Carlos CroccoCaliari, Marcelo VidigalCarneiro, Cláudia MartinsDiniz, Lívia de FigueiredoSilva, Eduardo de Almeida Marques daCaldas, Ivo SantanaMatta, Maria Adelaide do ValleSouza, Sheler Martins deLana, Marta deChiari, EglerGalvão, Lúcia Maria da CunhaBahia, Maria Terezinha2012-09-192012-09-192009GUEDES, P. M. da M. et al. Development of chronic cardiomyopathy in canine Chagas disease correlates with high IFN-g, TNF-a, and low IL-10 production during the acute infection phase. Veterinary Immunology and Immunopathology, v. 130, n. 1–2, p. 43–52, jul. 2009. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0165242709000221>. Acesso em: 19 set. 2012.01652427http://www.repositorio.ufop.br/handle/123456789/1399When infected with Trypanosoma cruzi, Beagle dogs develop symptoms similar to those of Chagas disease in human beings, and could be an important experimental model for a better understanding of the immunopathogenic mechanisms involved in chronic chagasic infection. This study evaluates IL-10, IFN-g and TNF-a production in the sera, culture supernatant, heart and cervical lymph nodes and their correlation with cardiomegaly, cardiac inflammation and fibrosis in Beagle dogs infected with T. cruzi. Pathological analysis showed severe splenomegaly, lymphadenopathy and myocarditis in all infected dogs during the acute phase of the disease, with cardiomegaly, inflammation and fibrosis observed in 83% of the animals infected by T. cruzi during the chronic phase. The data indicate that infected animals producing IL-10 in the heart during the chronic phase and showing high IL-10 production in the culture supernatant and serum during the acute phase had lower cardiac alterations (myocarditis, fibrosis and cardiomegaly) than those with high IFN-g and TNF-a levels. These animals produced low IL-10 levels in the culture supernatant and serum during the acute phase and did not produce IL-10 in the heart during the chronic phase of the disease. Our findings showed that Beagle dogs are a good model for studying the immunopathogenic mechanism of Chagas disease, since they reproduce the clinical and immunological findings described in chagasic patients. The data suggest that the development of the chronic cardiac form of the disease is related to a strong Th1 response during the acute phase of the disease, while the development of the indeterminate form results from a blend of Th1 and Th2 responses soon after infection, suggesting that the acute phase immune response is important for the genesis of chronic cardiac lesions.en-USTrypanosoma cruziCytokinesChagas' diseaseChronic cardiomyopathyDogsArtigo publicado em periodicoO periódico Veterinary Immunology and Immunopathology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3281420372149.