Browsing by Author "Bambirra, Eduardo Alves"
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Item American trypanosomiasis (chagas' disease) in conventional and germfree rats and mice.(1987) Silva, Marcelo Eustáquio; Evangelista, Elísio Alberto; Nicoli, Jacques Robert; Bambirra, Eduardo Alves; Vieira, Enio CarlosItem Coinfection with Toxoplasma gondii inhibits antigen-specific Th2 immune responses, tissue inflamation, and parasitism in BALB/c mice infected with Leishmania major.(1999) Santiago, Helton da Costa; Oliveira, Milton Adriano Pelli de; Bambirra, Eduardo Alves; Faria, Ana Maria Caetano de; Afonso, Luís Carlos Crocco; Oliveira, Leda Quercia; Gazzinelli, Ricardo TostesLesion size, cellular infiltration, and tissue parasitism in the footpads of BALB/c mice infected with Leishmania major were all dramatically inhibited during acute but not chronic infection with Toxoplasma gondii. Similarly, acute but not chronic toxoplasmosis at the time of infection with L. major had a strong inhibitory effect on development of acquired immune responses mediated by Th2 lymphocytes. In contrast, no mItem Effect of dietary chitin on cholesterol absorption and metabolism in rats.(1992) Zacour, Albertina C.; Silva, Marcelo Eustáquio; Cecon, Paulo Roberto; Bambirra, Eduardo Alves; Vieira, Enio CarlosThe effect of chitin at the level of 5% in the diet on cholesterol absorption and metabolism was studied in Wistar rats fed on diet containing beef tallow (7%) and cholesterol (1%). When compared with pair-fed controls, rats fed on diet containing chitin had: (1) similar weight gain and feed efficiency, (2) lower apparent protein digestibility, (3) equivalent liver steatosis, (4) reduced levels of liver triglycerides and cholesterol, (5) similar levels of serum and fecal cholesterol, (6) higher excretion of triglycerides in feces.Item Fase crônica cardíaca fibrosante da Tripanossomíase cruzi experimental no cão.(1988) Lana, Marta de; Tafuri, Washington Luiz; Caliari, Marcelo Vidigal; Bambirra, Eduardo Alves; Chiari, Cléa de Andrade; Leite, Virginea Hora Rios; Barbosa, Alfredo José Afonso; Toledo, Max Jean de Ornelas; Chiari, EglerDe acordo com os trabalhos publicados ate o momento, o cão esta sendo considerado, com ressalvas, como modelo ideal para o estudo da fase aguda e crônica indeterminada da tripanossomiase cruz jl 2 3 4 5 6 7 14 15 18 19 20 21 24 Os requisitos para um modelo ideal, estabelecidos pelo Comite de Doenca de Chagas do Programa Especial de Treinamento e Pesquisa de Doenças Parasitarias da Organização Mundial de Saude25 podem ser assim discriminados: permitir o isolamento do parasito ao longo do curso da infecção; apresentar reações sorológicas positivas, indicativas da persistência da infecção; apresentar manifestações clinicas da doença de Chagas crônica; desenvolver miocardite, miosite e outras alterações patológicas que caracterizam a doença; induzir a resposta imune contra tecido do hospedeiro. Há mais de oito anos estamos a procura de um modelo que não somente preencha todos os requisitos acima citados mas, principalmente, que desenvolva a cardiopatia grave evolutiva fibrosante com todas alterações clinicas observadas na forma humana. Ate o momento, os resultados que encontramos parecem indicar que alcançamos tal objetivo no modelo cão. A partir destes resultados e dos de outros autores, tentaremos aplicar metodologia moderna no estudo dos vários fatores patogeneticos no pressuposto de que, assim, será possível chegar ao esclarecimento da patogenia e de fisiopatologia das diferentes formas anatomoclinicas da doença. Dentre os numerosos fatores patogeneticos ate agora aventados, a fibrose nos parece o mais importante na determinação da insuficiência cardíaca congestiva (ICC) e da aperistalse. Não existe qualquer outra cardiopatia e/ou mega com aspecto tão peculiar. No miocárdio bem como nos megas, a fibrose (fibrilopoese) e focal e difusa ao mesmo tempo23. O presente trabalho tem a finalidade de documentar a fase crônica da doença de Chagas em cães que recebem inóculos diversos das cepas Colombiana13 e Berenice-7817 de T. cruzi, destacando aqueles animais que desenvolveram a cardiopatia fibrosante, com sinais e sintomas clínicos de ICC.Item Intracellular signal triggered by cholera toxin in Saccharomyces boulardii and Saccharomyces cerevisiae.(1998) Brandão, Rogélio Lopes; Castro, Ieso de Miranda; Bambirra, Eduardo Alves; Amaral, Sheila Coutinho; Fietto, Luciano Gomes; Trópia, Maria José Magalhães; Neves, Maria José; Santos, Raquel Gouvêa dos; Gomes, Newton Carlos Marcial; Nicoli, Jacques RobertAs is the case for Saccharomyces boulardii, Saccharomyces cerevisiae W303 protects Fisher rats against cholera toxin (CT). The addition of glucose or dinitrophenol to cells of S. boulardii grown on a nonfermentable carbon source activated trehalase in a manner similar to that observed for S. cerevisiae. The addition of CT to the same cells also resulted in trehalase activation. Experiments performed separately on the A and B subunits of CT showed that both are necessary for activation. Similarly, the addition of CT but not of its separate subunits led to a cyclic AMP (cAMP) signal in both S. boulardii and S. cerevisiae. These data suggest that trehalase stimulation by CT probably occurred through the cAMP-mediated protein phosphorylation cascade. The requirement of CT subunit B for both the cAMP signal and trehalase activation indicates the presence of a specific receptor on the yeasts able to bind to the toxin, a situation similar to that observed for mammalian cells. This hypothesis was reinforced by experiments with 125I-labeled CT showing specific binding of the toxin to yeast cells. The adhesion of CT to a receptor on the yeast surface through the B subunit and internalization of the A subunit (necessary for the cAMP signal and trehalase activation) could be one more mechanism explaining protection against the toxin observed for rats treated with yeasts.Item Mesangial C4d deposition may predict progression of kidney disease in pediatric patients with IgA nephropathy.(2017) Fabiano, Rafaela Cabral Gonçalves; Araújo, Stanley de Almeida; Bambirra, Eduardo Alves; Oliveira, Eduardo Araújo; Silva, Ana Cristina Simões e; Pinheiro, Sérgio Veloso BrantBackground Data on the risk factors for chronic kidney disease in children with immunoglobulin A nephropathy (IgAN) are scarce. This study was aimed at investigating whether glomerular C4d immunostaining is a prognostic marker in pediatric IgAN. Methods In this retrospective cohort study, 47 patients with IgAN biopsied from 1982 to 2010 were evaluated. Immunohistochemistry for C4d was performed in all cases. For analysis, patients were grouped according to positivity or not for C4d in the mesangial area. Primary outcome was a decline in baseline estimated glomerular filtration rate (eGFR) by 50% or more. Results Median follow-up was 8.3 years. Median renal survival was 13.7 years and the probability of a 50% decline in eGFR was 13% over 10 years. Nine children exhibited the primary outcome and 4 developed end-stage renal disease (ESRD). Compared with C4d-negative patients (n = 10), C4d-positive patients (n = 37) presented higher baseline proteinuria (1.66 ± 0.68 vs 0.47 ± 0.19 g/day/1.73 m2, p < 0.001), a progressive decline in eGFR (−10.04 ± 19.38 vs 1.70 ± 18.51 ml/min/1.73 m2/year; p = 0.045), and more frequently achieved the primary outcome (50.0 vs 10.8%, p = 0.013), and ESRD (30.0 vs 2.7%, p = 0.026). No difference was observed in Oxford classification variables. Baseline proteinuria, endocapillary hypercellularity and mesangial C4d deposition were associated with primary outcome in univariate analysis. Proteinuria and mesangial C4d deposition at baseline independently predicted the decline in eGFR. Renal survival was significantly reduced in C4d-positive patients (8.6 vs 15.1 years in C4d-negative patients, p < 0.001). Conclusions In this exclusively pediatric cohort, positivity for C4d in the mesangial area was an independent predictor of renal function deterioration in IgAN.Item The effect of chronic ingestion of ethanol on modulation of granulomatous inflammation in experimental schistosomiasis in mice.(1993) Castro, Lúcia Porto Fonseca de; Bambirra, Eduardo Alves; Coelho, Paulo Marcos Zech; Silva, Marcelo EustáquioWe studied the role of ethanol on the modulation of liver granulomata around Schistosoma mansoni eggs in mice. Albino mice, receiving 7% ethanol as the sole drinking liquid, at 60 and 90 days post-infection, presented smaller granulomata than controls did, when sacrificed at 120 days post-infection. No differences in diameters could observed, when ethanol was given 4 months before up to 120 days after infection. The results suggested that modulation of schistosomose granulomata by ethanol ingestion varies with time and duration of drug consumption.Item The effect of iron nutritional status on Trypanosoma cruzi infection in germfree and conventional mice.(1993) Pedrosa, Maria Lúcia; Nicoli, Jacques Robert; Silva, Marcelo Eustáquio; Silva, Marcio Eustáquio; Silva, Marcílio Eustáquio de Castro; Vieira, Leda Quercia; Bambirra, Eduardo Alves; Vieira, Enio Carlos1. Conventional (CV) and gnotobiotic (GN) female CFW mice were infected with the Y strain of Trypanosoma cruzi. 2. After infection, both CV and GN groups received injections of iron-dextran or desferrioxamine. Non-injected mice served as controls. 3. The parasitemia was more intense in iron-dextran-treated mice. 4. The iron levels in serum, liver, and spleen were: (a) not decreased by desferrioxamine and (b) increased by iron-dextran treatments. 5. An increase in leukocyte numbers was observed in all GN and CV groups after infection. 6. There was no difference in total iron binding capacity (TIBC) and iron saturation transferrin (IST) between GN and CV mice before infection. 7. In CV groups, after infection, TIBC was decreased whereas the levels of IST were increased; in GN the opposite occurred. 8. Trypanosome-specific IgG and IgM antibody levels were raised in the GN group but not in the CV group.Item The Oxford Classification predictors of chronic kidney disease in pediatric patients with IgA nephropathy.(2017) Fabiano, Rafaela Cabral Gonçalves; Araújo, Stanley de Almeida; Bambirra, Eduardo Alves; Oliveira, Eduardo Araújo; Silva, Ana Cristina Simões e; Pinheiro, Sérgio Veloso BrantObjective: The Oxford Classification for Immunoglobulin A nephropathy (IgAN) identifies pathological variables that may predict the decline of renal function. This study aimed to evaluate the Oxford Classification variables as predictors of renal dysfunction in a cohort of Brazilian children and adolescents with IgAN. Methods: A total of 54 patients with IgAN biopsied from 1982 to 2010 were assessed. Biopsies were re-evaluated and classified according to the Oxford Classification. Multivariate analysis of laboratory and pathological data was performed. The primary outcomes were decline of baseline estimated glomerular filtration rate (eGFR) greater than or equal to 50%. Results: Mean follow-up was 7.6 ± 5.0 years. Mean renal survival was 13.5 ± 0.8 years and probability of decline ≥50% in baseline eGFR was 8% at five years of follow-up and 15% at ten years. Ten children (18.5%) had a decline of baseline eGFR ≥ 50% and five (9.3%) evolved to end-stage renal disease. Kaplan---Meier analysis showed that baseline proteinuria, proteinuria during follow-up, endocapillary proliferation, and tubular atrophy/interstitial fibrosis were associated with the primary outcome. Multivariate Cox analysis showed that only baseline proteinuria (HR, 1.73; 95% CI, 1.20---2.50, p = 0.003) and endocapillary hypercellularity (HR, 37.18; 95% CI, 3.85---358.94, p = 0.002) were independent predictors of renal dysfunction. No other pathological variable was associated with eGFR decline in the multivariate analysis.Item Vitamin D overload and experimental Trypanosoma cruzi infection : parasitological and histopathological aspects.(1993) Silva, Marcelo Eustáquio; Silva, Marcílio Eustáquio de Castro; Nicoli, Jacques Robert; Bambirra, Eduardo Alves; Vieira, Enio Carlosl. Six groups of 45-day-old, 23.0 & 1.7 g, female Balb/c mice were inoculated intraperitoneally with 63, 252, 440, 630, 2520 or 6300 I.U. of vitamin D for 6 days. A seventh group was inoculated with saline. Each group consisted of 30 animals. 2. All animals inoculated with the doses of 2520 and 6300 and 70% of mice which received 630 I.U. of vitamin D died 21 days after the first administration of the vitamin. The LDm was 630 I.U. 3. The survivors were divided into two groups inoculated intraperitoneally with 5000 trypomastigotes of either Y or CL strain of Trypanosoma cruzi. 4. Based on the survival index on day 73 after infection, Vitamin D gave statistically significant protection (P < 0.01) for mice inoculated with doses of 63 or 430 I.U. of Y or CL strains, respectively. 5. On histopathological examination, inflammatory reaction and cellular and tissue parasitism were less intense in animals which received higher doses of vitamin D. 6. It is concluded that an overload of vitamin D had a protective effect against CL and Y strains of Trypanosoma cruzi infection in Balb/c mice.