Browsing by Author "Ferreira, Adaliene Versiani Matos"

Now showing 1 - 7 of 7
  • No Thumbnail Available
    Item
    Aerobic training reduces immune cell recruitment and cytokine levels in adipose tissue in obese mice.
    (2018) Lacerda, Débora Romualdo; Moraes, Michele Macedo; Silva, Albená Nunes da; Costa, Kátia Anunciação; Rodrigues, Débora Fernandes; Sabino, Josiana Lopes; Cordeiro, Letícia Maria de Souza; Pinho, Vanessa; Teixeira, Mauro Martins; Wanner, Samuel Penna; Soares, Danusa Dias; Ferreira, Adaliene Versiani Matos
    Obesity is associated with an energy imbalance that results from excessive energy intake, low diet quality and a sedentary lifestyle. In this regard, the increased consumption of a high-refined carbohydrate diet (HC) is strongly related to higher adiposity and low-grade inflammation. Aerobic training is a well-known non-pharmacological intervention to treat obesity and metabolic disturbances. However, the mechanisms through which aerobic training ameliorates the low-grade inflammation induced by the HC diet need to be further investigated. Herein, our hypothesis was that aerobic training would decrease the recruitment of leukocytes in the adipose tissue thereby reducing the levels of cytokines and improving metabolism in mice fed the HC diet. Male Balb/c were assigned to the following groups: control non-trained (C-NT), control trained (C-T), HC-NT and HC-T. Mice were submitted to moderate-intensity training sessions that consisted of running 60 min/day for 8 weeks. The intravital microscopy technique was performed in vivo in anesthetized mice to visualize the microvasculature of the adipose tissue. The HC diet induced obesity and increased the influx of immune cells into the adipose tissue. In contrast, HC-T mice presented a lower adiposity and adipocyte area. Furthermore, HC-T mice showed an increased resting energy expenditure, a decreased recruitment of immune cells in the adipose tissue, reduced cytokine levels, and ameliorated hyperglycemia and fatty liver deposition relative to HC-NT mice. Collectively, our data enhance the understanding about the antiinflammatory effect of aerobic training and shed light on the adipose tissue-mediated mechanisms by which training promotes a healthier metabolic profile.
  • No Thumbnail Available
    Item
    Efeito do tratamento com suco de uvaia (Eugenia uvalha Cambess) sobre a esteatose hepática e os mecanismos envolvidos no metabolismo de lipídios e estresse oxidativo em um modelo experimental.
    (2018) Lopes, Juliana Márcia Macedo; Silva, Marcelo Eustáquio; Pedrosa, Maria Lúcia; Ferreira, Adaliene Versiani Matos; Costa, Daniela Caldeira; Peluzio, Maria do Carmo Gouveia; Lima, Wanderson Geraldo de; Pedrosa, Maria Lúcia
    O consumo excessivo de uma dieta hiperlipídica eleva os níveis dos lipídios plasmáticos, podendo resultar em doença hepática gordurosa não alcoólica (NAFLD). Por outro lado, sabe-se que a dieta desempenha um papel fundamental na NAFLD, tornando as intervenções dietéticas para a prevenção um tópico importante para pesquisas particularmente devido à grande diversidade de compostos bioativos presentes nos alimentos. A uvaia é um fruto pertencente à família da Mirtaceae e vem sendo estudado por apresentar elevada capacidade antioxidante in vitro e por possuir diversos compostos bioativos relacionados a efeitos benéficos à saúde. Assim, o presente trabalho investigou o efeito do tratamento com suco de uvaia sobre a esteatose hepática e mecanismos envolvendo o metabolismo de lipídios e estresse oxidativo em um modeloexperimental de NAFLD induzida por dieta hiperlipídica. Trinta e dois ratos Ficsher fêmeas foram divididos inicialmente em 2 grupos experimentais, um grupo (C) recebeu dieta padrão AIN-93M, e o outro grupo (H) recebeu uma dieta hiperlipídica (25% de óleo de soja e 2% de colesterol) por duas semanas, após este período, os grupos C e H foram subdivididos em quatro grupos de oito animais de acordo com o tratamento recebido. Os grupos C e CUv receberam dieta padrão, e os grupos H e HUv receberam dieta hiperlipídica, os grupos CUv e HUv receberam tratamento com suco de uvaia (2 ml), administrado diariamente via gavagem por mais seis semanas. A administração do suco de uvaia reduziu significativamente o colesterol total e o colesterol não-HDL, reduziu o conteúdo de lipídios hepáticos em 23%, diminuiu o volume das macrovesículas de gordura e melhorou os níveis séricos das transaminases, apresentando, portanto, efeito protetor sobre a esteatose hepática. Além disso a administração do suco de uvaia aumentou a expressão do gene relacionado a β-oxidação de ácidos graxos mediada por PPAR-α, e modulou seu gene alvo ACOX1, diminui a expressão do gene da ácido graxo sintase. A uvaia ainda restaurou a biogênese peroxissomal quando observado a expressão de PEX11α, PMP70, genes relacionados com a proliferação do peroxissomo, e a catalase, principal enzima dessa organela. E ainda, promoveu uma melhora no balanço oxidante/antioxidante hepático quando observado os níveis de proteína carbonilada, glutationa reduzida, atividade de catalase e atividade paraoxonásica da enzima paraoxonase sugerindo uma proteção contra a progressão da doença. Esses achados sugerem o uso do fruto da uvaia como uma potencial terapia para injúrias hepáticas através da regulação da biogênese peroxisomal e do estresse oxidativo, apoiando a ideia de que os compostos bioativos são importantes na a prevenção da NAFLD.
  • No Thumbnail Available
    Item
    Impact of a physical activity program on plasma concentrations of adiponectin, leptin and ghrelin in overweight and obese schoolchildren : a randomized controlled trial.
    (2013) Freitas, André Everton de; Lamounier, Joel Alves; Soares, Danusa Dias; Oliveira, Thiago Henrique de; Lacerda, Débora Romualdo; Andrade, Joyce Batista; Komatsuzaki, Flávia; Botion, Leida Maria; Ferreira, Adaliene Versiani Matos; Silva, Camilo Adalton Mariano da; Rezende, Dilermando Fazito
    Introduction: Child obesity has important consequences on the child’s health and is related to the risk of developing chronic diseases. That brings up the need for research assessing the impact of obesity - and overweight - reduction programs with close attention to methodology aspects and precise indicators of the impact of such programs. Goal: Assessing the impact of a physical activity program on the plasma concentrations of adiponectin, leptin, and ghrelin in overweight and obese schoolchildren. Methods: Randomized controlled trial, double-blind, with 6- to 9-year-old overweight and obese school- children. A sample of 79 schoolchildren ran- domly distributed into control and intervention groups was subjected to a physical activity program. The levels of adiponectin, leptin, ghrelin, body mass index, body fat percentage, an- thropometry, lipid profile and glycemia were evaluated. The program’s impact was assessed through multivariate linear regression models. Result: When comparing the groups, a significant difference was found for adiponectin, which shows an increase in the proportion of subjects with higher adiponectin concentrations for the intervention group and a reduction in body mass index and body fat percentage. Conclusion: The impact of the intervention program was positive in increasing adiponectin levels associated with a reduction in body mass index and body fat percentage.
  • No Thumbnail Available
    Item
    Mechanisms underlying fat pad remodeling induced by fasting : role of PAF receptor.
    (2019) Lacerda, Débora Romualdo; Soares, Danusa Dias; Costa, Kátia Anunciação; Silva, Albená Nunes da; Rodrigues, Débora Fernandes; Sabino, Josiana Lopes; Silveira, Ana Letícia Malheiros; Pinho, Vanessa; Vieira, Érica Leandro Marciano; Menezes, Gustavo Batista; Antunes, Maísa Mota; Teixeira, Mauro Martins; Ferreira, Adaliene Versiani Matos
    Objectives: Fasting has long been practiced for political and religious reasons and to lose weight. However, biological responses during fasting have yet to be fully understood. Previous studies have shown that cytokines may control fat pad expansion, at least in part, owing to the induction of lipolysis. Indeed, we have previously shown that mice with a lower inflammatory response, such as platelet-activating factor receptor knockout mice (PAFR / ), are prone to gain weight and adiposity. The aims of this study were to determine whether adipose tissue becomes inflamed after fasting and to evaluate whether the PAF signaling is a fator in the fat loss induced by fasting. Methods: Wild-type (WT) and PAFR / mice were fasted for 24 h. Adiposity, leukocyte recruitment, and cytokine levels were evaluated. Multiple comparisons were performed using two-way analysis of variance and post hoc Fisher exact test. Results: After fasting, male WT mice showed lower adiposity (P < 0.001), higher recruitment of immune cells (P < 0.001), and increased cytokine levels (P < 0.05) in adipose tissue. Although WT mice lost ~79% of their adipose tissue mass, PAFR / mice lost only 36%. Additionally, PAFR / mice did not show enhanced cytokine and chemokine levels after fasting (P > 0.05). Conclusion: Despite low-grade inflammation being associated with metabolic syndrome, at least in part, the inflammatory milieu is also important to induce proper fat mobilization and remodeling of adipose tissue.
  • No Thumbnail Available
    Item
    Role of adipose tissue inflammation in fat pad loss induced by fasting in lean and mildly obese mice.
    (2019) Lacerda, Débora Romualdo; Costa, Kátia Anunciação; Silveira, Ana Letícia Malheiros; Rodrigues, Débora Fernandes; Silva, Albená Nunes da; Sabino, Josiana Lopes; Pinho, Vanessa; Menezes, Gustavo Batista; Soares, Danusa Dias; Teixeira, Mauro Martins; Ferreira, Adaliene Versiani Matos
    Inflammation induced by obesity contributes to insulin resistance and atherosclerosis. Indeed, high levels of proinflammatory cytokines trigger chronic lowgrade inflammation and promote detrimental metabolic effects in the adipose tissue. On the other hand, inflammation seems to control fat pad expansion and to have important functions on lipolysis and glucose metabolism. Thus, it is possible that inflammation may also drive fat pad loss, as seen during long-fast periods. Herein, we have used fasting as a strategy to induce weight loss and evaluate the possible role of inflammation on adipose tissue remodeling. Male BALB-c mice were fed with chow diet (lean mice) or with high-carbohydrate refined diet (mildly obese mice) for 8 weeks. After that, animals were subjected to 24 h of fasting. There was a 63% reduction of adiposity in lean mice following fasting. Furthermore, the adipose tissue was enriched of immune cells and had a higher content of IL-6, TNF-alpha, IL-10, TGF-β and CXCL-1. Interestingly, mildly obese mice, subjected to the same 24-h fasting period, lost only 33% of their adiposity. Following fasting, these mice did not show any increment in leukocyte recruitment and cytokine levels, as did lean mice. Our findings indicate that inflammation participates in fat mass loss induced by fasting. Although the chronic low-grade inflammation seen in obesity is associated with metabolic diseases, a lower inflammatory response triggered by fasting in mildly obese mice impairs fat pad mobilization.
  • No Thumbnail Available
    Item
    The transtheoretical model is an effective weight management intervention : a randomized controlled trial.
    (2020) Freitas, Patrícia Pinheiro de; Menezes, Mariana Carvalho de; Santos, Luana Caroline dos; Pimenta, Adriano Marçal; Ferreira, Adaliene Versiani Matos; Lopes, Aline Cristine Souza
    Background: Given the current worldwide epidemic of obesity, there is a demand for interventions with higher impact, such as those carried out in the primary health care (PHC) setting. Here we evaluate the effect of intervention performed according to the stages of change of the transtheoretical model (TTM) for weight management. Methods: This randomized controlled trial in Brazilian PHC offered free physical exercise and nutrition education. The participants were women, aged 20 years or older who were obese or overweight, users in PHC service. The intervention group (IG, n = 51) received the same orientation as the comparison group (CG, n = 35) plus individual health counseling based on the TTM aimed at weight loss, which lasted 6 months. The outcome measures were anthropometric, food, and nutrient profiles. Inflammatory parameters were evaluated in a random subsample. The inter-group and intra-group differences were evaluated using interntion-to-treat analysis, and analysis of covariance (ANCOVA) used to assess intervention effectiveness. Results: There was a difference between groups of − 1.4 kg (CI95%: − 2.5; − 0.3) in body weight after the intervention. About 97% of women in the IG reported benefits of the intervention and presented positive changes in diet, biochemical markers, and anthropometry. The IG showed better body mass index, resistine, and blood glucose results compared to the CG during follow-up. Conclusion: The individualized TTM-based intervention, combined with usual care, was an effective strategy in PHC. These results should encourage the use of interdisciplinary practices; nevertheless, research to identify additional strategies is needed to address barriers to weight maintenance among obese low-income women. Trial registration: The trial is registered with Brazilian clinical trials under the code: RBR-8t7ssv, Registration date: 12/12/2017 (retrospectively registered).
  • No Thumbnail Available
    Item
    Validation of metabolic syndrome and its self reported components in the CUME study.
    (2017) Miranda, Aline Elizabeth da Silva; Ferreira, Adaliene Versiani Matos; Oliveira, Fernando Luiz Pereira de; Hermsdorff, Helen Hermana Miranda; Bressan, Josefina; Pimenta, Adriano Marçal
    The aim of this study was to analyze the validity of self-reported diagnoses of metabolic syndrome (MetS) and its components through participants of the Cohort of Universities of Minas Gerais (CUME). A subsample of 172 cohort participants (33 males and 139 females, age 38 ± 11 years) was randomly selected for this study. The presence of MetS was defined according to the criteria of the International Diabetes Federation (IDF). Data on weight, height, blood pressure, and serum concentration of glucose, triglycerides and HDL-c were self reported in an online cohort questionnaire, and the same variables were measured using a standardized protocol in laboratories of higher education institutions involved in the project. Self-reported and measured data were compared by means of intraclass correlation coefficient (ICC), Kappa coefficient (k) and differences between self-reported and measured data, according to the Bland and Altman method. The prevalence of MetS was 4.7% and 5.2% according to self-reported and measured data, respectively. The Kappa coefficient between diagnoses of self-reported and measured MetS was 0.814, indicating almost perfect agreement, a situation similar to that observed for obesity (k = 0.882). The other components of MetS had moderate agreement (k = 0.41 to 0.60). The ICC also indicated excellent agreement for weight, height, BMI and HDL-c, respectively, 0.989, 0.995, 0.983 and 0.761. Glucose presented low agreement (ICC: 0.366). The study concludes that the CUME project participants provided valid information for the self-reported diagnoses of MetS and its components.