Browsing by Author "Kamino, Luciana Hiromi Yoshino"
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Item Alcaligenes faecalis associated with Mimosa calodendron rizhosphere assist plant survival in arsenic rich soils.(2017) Felestrino, Érica Barbosa; Assis, Renata de Almeida Barbosa; Lemes, Camila Gracyelle de Carvalho; Cordeiro, Isabella Ferreira; Fonseca, Natasha Peixoto; Villa, Morghana Marina; Vieira, Izadora Tabuso; Kamino, Luciana Hiromi Yoshino; Carmo, Flávio Fonseca do; Moreira, Leandro MarcioThe ferruginous rupestrian grasslands (FRG) in the Iron Quadrangle (IQ) are ecosystems characterized by rocky soils with reduced availability of water and nutrients, but high levels of metals. In order to comprehend the interference of microorganisms on the adaptive process of endemic plant Mimosa calodendrum (Fabaceae), bacteria associated with its roots and rhizosphere were isolated. Fourteen isolates were obtained and subsequently grown in the presence of different concentrations of arsenic (As) species. The isolate Mc250, an Alcaligenes faecalis strain, resisted to 10 mM of As (III) and 800 mM of As (V). In the presence of this strain, atomic spectrometer detected a reduction of 55% for As (III) and 72% for As (V) respectively in 10 mM and 500 mM solution. Scanning electron microscopy of this isolate demonstrated morphological modification and EDX spectroscopy revealed the presence of both As species adsorbed on the membrane, justifying the removal observed in the in vitro assays. To validate this potential removal of As in vivo, tomato plants were used as grown model in the presence and absence of A. faecalis in soil previously contaminated with 5 mM of As (III). After 14 days, plants from contaminated soil had their growth improved when compared to untreated control plants. All these results suggest for the first time that plant-associated bacteria from FRG-IQ present potential for soil rhizoremediation and may benefit the adaptive processes of plants in extreme environments including application in recovering degraded areas.Item Atlas digital geoambiental como instrumento de educação ambiental nas escolas públicas de Minas Gerais.(2017) Reis, Luiz Henrique de Oliveira; Moreira, Leandro Marcio; Kamino, Luciana Hiromi Yoshino; Moreira, Leandro Marcio; Kamino, Luciana Hiromi Yoshino; Cruz, Luciana Hoffert CastroNa relação do ensino e aprendizagem acredita-se que a concepção de que as novas Tecnologias da Informação e Comunicação - TICs devem reformular a Metodologia Educacional atual, leva a buscar de que maneira deve-se pensar e agir para entender e compreender esta nova estrutura, estrutura está aberta, complexa, sem hierarquias. Aprender as novas formas de raciocínio, de organização e representação do pensamento não é tarefa fácil. As inadequações dos modelos existentes de transmissão do conhecimento, processos pedagógicos, dificultam em muito a aplicação no ensino e aprendizagem. O que se observa é que as mudanças sócio-econômico-culturais geradas pela Tecnologia da Informação e Comunicação - TIC causaram grandes mudanças nos alicerces da Educação, exigindo transformações, na estrutura e nos elementos da educação escolar. Este estudo tem como objetivo analisar o uso das novas Tecnologias da Informação e Comunicação – TICs na Educação Ambiental, mais especificamente, analisar a eficácia da utilização do Atlas Digital Geoambiental como instrumento da Educação Ambiental e propor, se for o caso, seu uso no ensino e aprendizagem nas escolas de Minas Gerais. A principal hipótese deste estudo é que as novas Tecnologia de Informação e Comunicação – TICs, mais especificamente o Atlas Digital Geoambiental, pode provocar modificação na relação com o saber influenciando o funcionamento das instituições de Educação e a formação de profissionais de educação e dos alunos, no que diz respeito ao processo de ensino e aprendizagem. Conclui-se que o estudo surge como uma nova metodologia da educação para o ensino e aprendizagem com uso do Atlas Digital Geoambiental, surge a sala de aula virtual, funcionando em “escolas virtuais” tendendo a diminuição do período de aula, assim como a disponibilidade de acesso a aula 24 horas/dia. A força de trabalho que impulsiona esta nova metodologia é formada por professores que atuam com base na informação.Item Biotechnological potential of plant growth-promoting bacteria from the roots and rhizospheres of endemic plants in ironstone vegetation in southeastern Brazil.(2018) Felestrino, Érica Barbosa; Vieira, Izadora Tabuso; Caneschi, Washington Luiz; Cordeiro, Isabella Ferreira; Assis, Renata de Almeida Barbosa; Lemes, Camila Gracyelle de Carvalho; Fonseca, Natasha Peixoto; Sanchez, Angelica Bianchini; Caicedo Cepeda, Juan Carlos; Ferro, Jesus Aparecido; Garcia, Camila Carrião Machado; Carmo, Flávio Fonseca do; Kamino, Luciana Hiromi Yoshino; Moreira, Leandro MarcioMicroorganisms associated with plants have a great biotechnological potential, but investigations of these microorganisms associated with native plants in peculiar environments has been incipient. The objective of this study was to analyze the plant growth-promoting bacteria potential of cultivable bacteria associated with rare plants from the ferruginous rocky fields of the Brazilian Iron Quadrangle. The roots and rhizospheres of nine endemic plants species and samples of a root found in a lateritiric duricrust (canga) cave were collected, the culturable bacteria isolated and prospected for distinct biotechnological and ecological potentials. Out of the 148 isolates obtained, 8 (5.4%) showed potential to promote plant growth, whereas 4 (2.7%) isolates acted as biocontrol agents against Xanthomonas citri pathotype A (Xac306), reducing the cancrotic lesions by more than 60% when co-inoculated with this phytopathogen in Citrus sinensis plants. Moreover, other 4 (2.7%) isolates were classified as potential bioremediation agents, being able to withstand high concentrations of arsenite (5 mM As3+) and arsenate (800 mM As5+), by removing up to 35% and 15% of this metalloid in solution, respectively. These same four isolates had a positive influence on the growth of both the roots and the aerial parts when inoculated with tomato seeds in the soil contaminated with arsenic. This is the first time that an investigation highlights the potentialities of bacteria associated with rare plants of ferruginous rocky fields as a reservoir of microbiota of biotechnological and ecological interest, highlighting the importance of conservation of this area that is undergoing intense anthropic activityItem Brazilian ironstone plant communities as reservoirs of culturable bacteria with diverse biotechnological potential.(2018) Caneschi, Washington Luiz; Felestrino, Érica Barbosa; Fonseca, Natasha Peixoto; Villa, Morghana Marina; Lemes, Camila Gracyelle de Carvalho; Cordeiro, Isabella Ferreira; Assis, Renata de Almeida Barbosa; Sanchez, Angelica Bianchini; Vieira, Izadora Tabuso; Kamino, Luciana Hiromi Yoshino; Carmo, Flávio Fonseca do; Garcia, Camila Carrião Machado; Moreira, Leandro MarcioExtensive mineral extractivism in the Brazilian Iron Quadrangle (IQ) region has destroyed large areas of land, decimating plant species, and their associated microbiota. Very little is known about the microbiota of the region; hence, cultivable bacteria associated with plants of its soils were investigated for their biotechnological potential. Samples were collected from nine plant species and six soils, and 65 cultivable bacterial isolates were obtained. These represent predominantly gram-positive bacilli (70%) capable of producing amylases (55%), proteases (63%), cellulases (47%), indole acetic acid (IAA) (46%), siderophores (26%), and to solubilize phosphate (9%). In addition, 65% of these were resistant to ampicillin, 100% were sensitive to tetracycline, and 97% were tolerant to high arsenic concentrations. Three isolates were studied further: the isolate FOB3 (Rosenbergiella sp.) produced high concentrations of IAA in vitro in the absence of tryptophan – shown by the significant improvement in plant germination and growth rate where the isolate was present. For isolates C25 (Acinetobacter sp.) and FG3 (Serratia sp.), plasmids were purified and inserted into Escherichia coli cells where they modified the physiological profile of the transformed strains. The E. coli::pFG3B strain showed the highest capacity for biofilm production, as well as an increase in the replication rate, arsenic tolerance and catalase activity. Moreover, this strain increased DNA integrity in the presence of arsenic, compared to the wild-type strain. These results help to explain the importance of bacteria in maintaining plant survival in ferruginous, rocky soils, acting as plant growth promoters, and to highlight the biotechnological potential of these bacteria. IMPORTANCE The Iron Quadrangle region is responsible for ∼60% of all Brazilian iron production and, at the same time, is responsible for housing a wide diversity of landscapes, and consequently, a series of endemic plant species and dozens of rare species – all of which have been poorly studied. Studies exploring the microbiota associated with these plant species are limited and in the face of the continuous pressure of extractive action, some species along with their microbiota are being decimated. To understand the potential of this microbiota, we discovered that cultivable bacterial isolates obtained from plants in the ferruginous rocky soil of the Iron Quadrangle region have diverse biotechnological potential, revealing a genetic ancestry still unknown.Item Cangas ferruginosas : proposta pedagógica sobre a necessidade de conservação de um ecossistema ameaçado.(2016) Maciel, Jalula Maria Lage; Carmo, Flávio Fonseca do; Kamino, Luciana Hiromi Yoshino; Moreira, Leandro MarcioA educação ambiental (EA), enquanto campo epistemológico, pode auxiliar no despertar da compreensão do papel do indivíduo no ambiente. Neste trabalho, apresenta-se o resultado da parceria entre o Mestrado Profissional em Ensino de Ciências da Universidade Federal de Ouro Preto e o Instituto Prístino, com o objetivo de desenvolver uma cartilha de EA, envolvendo o ecossistema de canga, para professores e alunos do ensino fundamental II. Em Minas Gerais, os ecossistemas ferruginosos – conhecidos como “cangas” – apresentam características abióticas que resultaram em um dos ambientes com maior endemismo vegetal do Brasil. Atuando como reserva hídrica que abastece mananciais da região metropolitana de Belo Horizonte, as cangas estão na lista dos ecossistemas mais ameaçados, em especial pela mineração. Este artigo fornece conceitos, peculiaridades, importâncias, ameaças e desafios, além de exercícios de fixação e discussão contextualizada, sendo, portanto, uma ferramenta diferenciada para o ensino de Ciências.Item Complete genome sequence and analysis of Alcaligenes faecalis strain Mc250, a new potential plant bioinoculant.(2020) Felestrino, Érica Barbosa; Sanchez, Angelica Bianchini; Caneschi, Washington Luiz; Lemes, Camila Gracyelle de Carvalho; Assis, Renata de Almeida Barbosa; Cordeiro, Isabella Ferreira; Fonseca, Natasha Peixoto; Villa, Morghana Marina; Vieira, Izadora Tabuso; Kamino, Luciana Hiromi Yoshino; Carmo, Flávio Fonseca do; Silva, Aline Maria da; Thomas, Andrew Maltez; Patané, José Salvatore Leister; Ferreira, Fernanda Carla; Freitas, Leandro Grassi de; Varani, Alessandro de Mello; Ferro, Jesus Aparecido; Silva, Robson Soares; Almeida Junior, Nalvo Franco de; Garcia, Camila Carrião Machado; Setubal, João Carlos; Moreira, Leandro MarcioHere we present and analyze the complete genome of Alcaligenes faecalis strain Mc250 (Mc250), a bacterium isolated from the roots of Mimosa calodendron, an endemic plant growing in ferruginous rupestrian grasslands in Minas Gerais State, Brazil. The genome has 4,159,911 bp and 3,719 predicted protein-coding genes, in a single chromosome. Comparison of the Mc250 genome with 36 other Alcaligenes faecalis genomes revealed that there is considerable gene content variation among these strains, with the core genome representing only 39% of the protein-coding gene repertoire of Mc250. Mc250 encodes a complete denitrification pathway, a network of pathways associated with phenolic compounds degradation, and genes associated with HCN and siderophores synthesis; we also found a repertoire of genes associated with metal internalization and metabolism, sulfate/sulfonate and cysteine metabolism, oxidative stress and DNA repair. These findings reveal the genomic basis for the adaptation of this bacterium to the harsh environmental conditions from where it was isolated. Gene clusters associated with ectoine, terpene, resorcinol, and emulsan biosynthesis that can confer some competitive advantage were also found. Experimental results showed that Mc250 was able to reduce (~60%) the virulence phenotype of the plant pathogen Xanthomonas citri subsp. citri when co-inoculated in Citrus sinensis, and was able to eradicate 98% of juveniles and stabilize the hatching rate of eggs to 4% in two species of agricultural nematodes. These results reveal biotechnological potential for the Mc250 strain and warrant its further investigation as a biocontrol and plant growth-promoting bacterium.Item Ecocereus e as multifunções ecológicas da canga : contribuições para educação ambiental.(2016) Maciel, Jalula Maria Lage; Moreira, Leandro Marcio; Carmo, Flávio Fonseca do; Kamino, Luciana Hiromi Yoshino; Cruz, Luciana Hoffert CastroO modelo de produção a que assistimos desde meados do séc. XVIII, com a revolução industrial, não leva em consideração a finitude dos recursos naturais. Desta forma, a exploração do meio ambiente, não sustentável e que, portanto, não leva em conta a perda do habitat de diversas espécies e o desequilíbrio biológico, precisa ser debatida com todas s esferas da sociedade. Nesta ótica de discussão científica e de causas e efeitos, a escola entra como um setor fundamental, mas para isso necessita de uma pedagogia diferenciada e uma mudança de paradigma no processo de ensinar. Nesse ponto, a Educação ambiental mostra-se como excelente ferramenta para levar os indivíduos a compreenderem o seu papel enquanto parte de uma macro-natureza. Neste trabalho limitaremos a relação homem-natureza no contexto dos campos rupestres ferruginosos ou simplesmente canga, como são mais conhecidos. Localizados na região do Quadrilátero Ferrífero (QF) em Minas Gerais, estão entre os ecossistemas menos conhecidos e os mais ameaçados do Brasil, devido a sua distribuição restrita e associada aos principais depósitos de minério de ferro do país. Este trabalho apresenta as possibilidades didático-pedagógicas do uso da cartilha nomeada “Canga”, para o desenvolvimento de valores e atitudes em prol da conservação e preservação dos campos rupestres ferruginosos. Com a proposta de fazer da cartilha um instrumento de reflexão, um personagem (signo) protagonista da história norteou toda a apresentação das informações, um cacto endêmico de canga (Arthrocereus glaziovii) nomeado como Ecocereus. Trata-se de um Estudo de caso, em associação com análise documental numa abordagem qualitativa, desenvolvida com 37 alunos do 9º ano do ensino fundamental II de uma Escola Municipal do município de João Monlevade - MG. As etapas do estudo incluíram um questionário preliminar investigativo, seguido de distribuição e análise das informações presentes na cartilha, seguido de entrevista semi-estruturada, que permitiu analisar o envolvimento dos alunos com a proposta, bem como a qualidade das respostas dadas pelos mesmos em cada atividade proposta. Com base nos resultados obtidos ficou evidenciado que a cartilha além de ser única na proposta envolvendo a temática, representa uma ferramenta de apoio à prática docente no ensino fundamental, auxiliando no processo de inserção da dimensão ambiental, nas práticas pedagógicas, na construção de conhecimentos e na formação de valores e atitudes conscientes voltados à preservação e conservação desse ecossistema tão importante e ao mesmo tempo desconhecido da população. _______________________________________________________________________________________ ABSTRACT: The production model that we have seen since the XVIII mid-century, with the industrial revolution, it does not take into account the finiteness of natural resources. Thus, the environmental exploitation, unsustainable and therefore does not take into account habitat loss of many species and the biological imbalance needs be discussed with all s spheres of society. In this light of scientific discussion and causes and effects, the school enters as a key sector, but it needs a differentiated learning and a paradigm shift in the process of teaching. At this point, the Environmental education is shown as an excellent tool to bring individuals to understand their role as part of a macro-nature. This work will limit the man-nature relationship in the context of ferruginous rocky fields or simply sarong, as they are best known. Located in the Iron Quadrangle region (IQ) in Minas Gerais, they are among the least known ecosystems and the most threatened in Brazil, due to its restricted distribution and associated with major iron ore deposits in the country. This dissertation presents the didactic and pedagogical possibilities of using the primer named "Canga" for the development of values and attitudes towards the conservation and preservation of ferruginous rocky fields. With the proposal to make the booklet an instrument of reflection, a character (sign) protagonist of the story has guided the entire presentation of the information, an endemic cactus kanga (Arthrocereus glaziovii) named Ecocereus. This is a case study in association with document analysis in a qualitative approach, developed with 37 students from 9th grade of elementary school of a municipal school in the João Monlevade city - MG. The study's steps included an investigative preliminary questionnaire, followed by distribution and analysis of the information present in the primer, followed by semi-structured interview, which allowed qualitatively analyze the involvement of students with the proposal and the quality of the answers given by them in each proposed activity. Based on the results obtained it evidenced that the primer as well as being unique in the proposal involving the theme, is a teaching practice support tool in elementary school, assisting in the integration of the environmental dimension process in pedagogical practices, in building knowledge and in the formation of values and conscious attitudes aimed at preservation and conservation of this ecosystem so important and at the same time unknown population.Item Potential bioinoculants for sustainable agriculture prospected from ferruginous caves of the Iron Quadrangle/Brazil.(2021) Lemes, Camila Gracyelle de Carvalho; Cordeiro, Isabella Ferreira; Fernandes, Camila Henriques de Paula; Silva, Ana K.; Carmo, Flávio Fonseca do; Kamino, Luciana Hiromi Yoshino; Carvalho, Flávia M. S.; Caicedo Cepeda, Juan Carlos; Ferro, Jesus Aparecido; Moreira, Leandro MarcioBiocontrol and plant growth-promoting bacteria (PGPB) are important agricultural bioinoc- ulants. This study aimed to prospect new potential bioinoculants for a more sustainable agriculture from ferruginous caves of the Brazilian Iron Quadrangle. Culturable bacteria, from seven caves and one canga soil sample, were evaluated for biocontroller activity of the phytopathogens Xanthomonas citri subsp. Citri—Xcc306 (citrus canker), Fusarium oxysporum—Fo (fusariosis), and Colletotrichum lindemuthianum—Cl89 (bean anthracnose). The ability of the superior candidates to solubilize inor- ganic phosphate, fix nitrogen, and produce hydrolytic enzymes and siderophores was then analyzed. Out of 563 isolates, 47 inhibited the growth of Xcc306 in vitro, of which 9 reduced citrus canker up to 68% when co-inoculated with the pathogen on host plants. Twenty of the 47 inhibited Fo growth directly by 51–73%, and 15 indirectly by 75–81%. These 15 inhibited Cl89 growth in vitro (up to 93% directly and 100% indirectly), fixed nitrogen, produced proteases and siderophores, showed motility ability, produced biofilm, and all but one solubilized inorganic phosphate. Therefore, 15 (2.66%) bacterial isolates, from the genera Serratia, Nissabacter, and Dickeya, act simultaneously as biocontrollers and PGPBs, and could be important candidates for future investigations in planta as an alternative to minimize the use of pesticides and chemical fertilizers through sustainable agricultural management practices.Item Serratia liquefaciens FG3 isolated from a metallophyte plant sheds light on the evolution and mechanisms of adaptive traits in extreme environments.(2019) Caneschi, Washington Luiz; Sanchez, Angelica Bianchini; Felestrino, Érica Barbosa; Lemes, Camila Gracyelle de Carvalho; Cordeiro, Isabella Ferreira; Fonseca, Natasha Peixoto; Villa, Morghana Marina; Vieira, Izadora Tabuso; Moraes, Lauro Ângelo Gonçalves de; Assis, Renata de Almeida Barbosa; Carmo, Flávio Fonseca do; Kamino, Luciana Hiromi Yoshino; Silva, Robson Soares; Ferro, Jesus Aparecido; Ferro, Maria Inês Tiraboschi; Ferreira, Rafael Marini; Santos, Vera Lúcia; Silva, Ubiana de Cássia Mourão; Almeida Junior, Nalvo Franco de; Varani, Alessandro de Mello; Garcia, Camila Carrião Machado; Setubal, João Carlos; Moreira, Leandro MarcioSerratia liquefaciens strain FG3 (SlFG3), isolated from the flower of Stachytarpheta glabra in the Brazilian ferruginous fields, has distinctive genomic, adaptive, and biotechnological potential. Herein, using a combination of genomics and molecular approaches, we unlocked the evolution of the adaptive traits acquired by S1FG3, which exhibits the second largest chromosome containing the largest conjugative plasmids described for Serratia. Comparative analysis revealed the presence of 18 genomic islands and 311 unique protein families involved in distinct adaptive features. S1FG3 has a diversified repertoire of genes associated with Nonribosomal peptides (NRPs/PKS), a complete and functional cluster related to cellulose synthesis, and an extensive and functional repertoire of oxidative metabolism genes. In addition, S1FG3 possesses a complete pathway related to protocatecuate and chloroaromatic degradation, and a complete repertoire of genes related to DNA repair and protection that includes mechanisms related to UV light tolerance, redox process resistance, and a laterally acquired capacity to protect DNA using phosphorothioation. These findings summarize that SlFG3 is well-adapted to different biotic and abiotic stress situations imposed by extreme conditions associated with ferruginous fields, unlocking the impact of the lateral gene transfer to adjust the genome for extreme environments, and providing insight into the evolution of prokaryotes.