Browsing by Author "Oliveira, Juliana Velasco de Castro"
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Item Cachaça yeast strains : alternative starters to produce beer and bioethanol.(2018) Araújo, Thalita Macedo; Souza, Magalhaes Teixeira de; Diniz, Raphael Hermano Santos; Yamakawa, Celina Kiyomi; Soares, Lauren Bergmann; Lenczak, Jaciane Lutz; Oliveira, Juliana Velasco de Castro; Goldman, Gustavo Henrique; Barbosa, Edilene Alves; Campos, Anna Clara Silva; Castro, Ieso de Miranda; Brandão, Rogélio LopesThis work was performed to verify the potential of yeast strains isolated from cachaça distilleries for two specific biotechnological applications: beer and bioethanol production. In the beer production, the strains were tested for characteristics required in brewery practices, such as: capacity to ferment maltose and maltotriose, ability to grow at lowest temperatures, low H2S production, and flocculation profile. Among the strains tested, two of them showed appropriate characteristics to produce two different beer styles: lager and ale. Moreover, both strains were tested for cachaça production and the results confirmed the capacity of these strains to improve the quality of cachaça. In the bioethanol production, the fermentation process was performed similarly to that used by bioethanol industries: recycling of yeast biomass in the fermentative process with sulfuric acid washings (pH 2.0). The production of ethanol, glycerol, organic acids, dry cell weight, carbohydrate consumption, and cellular viability were analyzed. One strain presented fermentative parameters similar to PE2, industrial/commercial strain, with equivalent ethanol yields and cellular viability during all fermentative cycles. This work demonstrates that cachaça distilleries seem to be an interesting environment to select new yeast strains to be used in biotechnology applications as beer and bioethanol production.Item Draft genome sequence of Wickerhamomyces anomalus LBCM1105, isolated from cachaça fermentation.(2020) Cunha, Aureliano Claret da; Santos, Renato Augusto Corrêa dos; Pachón, Diego Mauricio Riaño; Squina, Fábio Márcio; Oliveira, Juliana Velasco de Castro; Goldman, Gustavo Henrique; Souza, Aline Tieppo de; Gomes, Lorena Soares; Santos, Fernanda Godoy; Teixeira, Janaina Aparecida; Oliveira, Fábio Faria; Cruz, Izinara Rosse da; Castro, Ieso de Miranda; Lucas, Cândida; Brandão, Rogélio LopesWickerhamomyces anomalus LBCM1105 is a yeast isolated from cachaça distillery fermentation vats, notable for exceptional glycerol consumption ability. We report its draft genome with 20.5x in-depth coverage and around 90% extension and completeness. It harbors the sequences of proteins involved in glycerol transport and metabolism.