Browsing by Author "Paulino Junior, Eduardo"

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    Pheochromocytoma-induced shock : a case report.
    (2012) Araújo, Stanley de Almeida; Carmo, Paula Alves Santos do; Paulino Junior, Eduardo; Borges, Isabela Nascimento; Rocha, Luiz Otávio Savassi
    Because of its rarity, together with the variability and nonspecificity of its signs and symptoms, pheochromocytoma, a tumor arising from chromaffin cells, creates an unlucky paradox: it is often missed but only rarely found. Besides the association with arterial hypertension, often in the form of paroxysmal attacks, pheochromocytoma may also be associated, in up to 40% of cases, with orthostatic hypotension which, when present, provides a clue to the diagnosis of the tumor. Far more rare (about 2% of cases) is the clinical presentation in the form of shock, a possibility that, among other attributes, justifies the epithet “the great mimic” applied to the neoplasia. The authors report the case of a 51-year-old hypertensive woman whose death was erroneously attributed to septic shock. Autopsy disclosed an unsuspected left adrenal bulky pheochromocytoma with areas of hemorrhage and extensive central necrosis, pronounced pulmonary edema, left ventricular mural thrombus, and histological evidence of acute myocardial injury.
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    Raman spectroscopy with a 1064-nm wavelength laser as a potential molecular tool for prostate cancer diagnosis : a pilot study.
    (2018) Magalhães, Felipe Lima; Machado, Alexei Manso Corrêa; Paulino Junior, Eduardo; Sahoo, Sangram K.; Paula, Ana Maria de; Garcia, Aloísio Miguel; Barman, Ishan; Soares, Jaqueline dos Santos; Lewer, Marcelo Henrique Mamede
    Raman spectroscopy is widely used to investigate the structure and property of the molecules from their vibrational transitions and may allow for the diagnosis of cancer in a fast, objective, and nondestructive manner. This experimental study aims to propose the use of the 1064-nm wavelength laser in a Raman spectroscopy and to evaluate its discrimination capability in prostate cancer diagnosis. Seventy-four spectra from patients who underwent radical prostatectomy were evaluated. The acquired signals were filtered, normalized, and corrected for possible oscillations in the laser intensity and fluorescence effects. Wilcoxon tests revealed significant differences between the benign and malign samples associated with the deformation vibration characteristic of nucleic acids, proteins, and lipids. A classifier based on support vector machines was able to predict the Gleason scores of the samples with 95% of accuracy, opening a perspective for the use of the 1064-nm excitatory wavelength in prostatic cancer diagnosis.
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    Second harmonic generation imaging of the collagen architecture in prostate cancer tissue.
    (2018) Garcia, Aloísio Miguel; Magalhães, Felipe Lima; Soares, Jaqueline dos Santos; Paulino Junior, Eduardo; Lima, Mario Felix Richard de; Lewer, Marcelo Henrique Mamede; Paula, Ana Maria de
    Optical microscopy has been one of the most important tools for visualizing biological samples since the seventeenth century. Recently, with the advances in femtosecond laser technology, all the nonlinear optical processes have now been included as optical microscopy methods, and second harmonic generation (SHG) microscopy has emerged as a powerful new optical imaging tool with applications in medicine and biology. Here we use SHG microscopy to obtain images of 76 prostate biopsies on histological slides. Multiple samples from the excised prostates of patients who underwent a radical prostatectomy were evaluated. The samples were collected from prostate positions as in needle biopsy procedures. The results show the collagen fiber architecture among malignant acini, and analysis of the fiber orientation in the images reveals that the collagen fibers become more aligned at higher malignancy grades. Furthermore, we find that the degree of fiber alignment correlates directly with the Gleason patterns.