Browsing by Author "Pereira, Andressa Rezende"

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    Analysis of tylosin in poultry litter by HPLC-UV and HPLC-MS/MS after LTPE.
    (2020) Paranhos, Aline Gomes de Oliveira; Pereira, Andressa Rezende; Fonseca, Isadora Chagas da; Sanson, Ananda Lima; Afonso, Robson José de Cássia Franco; Aquino, Sergio Francisco de
    The growing demand for chicken meat in Brazil encourages poultry producers to invest in antibiotics as growth promoters to ensure the quality of their products. Among these, the antibiotic tylosin is a widely used growth promoter that assists in the treatment of necrotic enteritis. Antibiotics usually are not totally metabolised and may be excreted in its original form, thereby contaminating different environmental compartments and may induce microbial resistance. Therefore, analyses of such contaminants in different matrices are important to perform risk assessments and this often depends on the development of extraction methods to allow the quantification of the substance at low concentrations. This work presents a simple and cheap methodology which employs low-temperature partitioning extraction (LTPE) for the analysis of tylosin in poultry litter samples by HPLC-UV and HPLC-MS/MS. The analytical method developed was validated according to Brazilian regulating agencies (ANVISA and MAPA) and resulted in method detection (LDM) and quantification (LQM) limits of 6.32 μg.kg−1 and 21.12 μg.kg−1, respectively. The analysis of the waste from a chicken broiler company, located in Minas Gerais, confirmed the adequacy of the method, since it detected tylosin at average concentrations of 135.22 μg.kg−1 and 264.92 μg.kg−1 in poultry litter from sheds containing birds in the rearing and production phase, respectively.
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    Biodegradation of sulfamethoxazole by microalgae-bacteria consortium in wastewater treatment plant effluents.
    (2020) Rodrigues, Daniel Aparecido da Silva; Cunha, Camila Cristina Rodrigues Ferreira da; Freitas, Mylena Gomes; Barros, André Luis Corrêa de; Castro, Paulo Bernardo Neves e; Pereira, Andressa Rezende; Silva, Silvana de Queiroz; Santiago, Aníbal da Fonseca; Afonso, Robson José de Cássia Franco
    Sulfamethoxazole (SMX) has been commonly detected in wastewater treatment plant (WWTP) effluents. SMX and other antibiotics can be considered as environmental contaminants of emerging concern. Due to their toxicity effects and their potential for the development of bacterial resistance their presence in aquatic compartment becomes a threat to human health. This study evaluated the bioremediation of SMX in WWTP effluents using a tertiary treatment composed by microalgae-bacteria consortium under low intensity artificial LED illumination, and also the assessment of sulfonamide resistance gene (sul1). The removal of SMX from WWTP effluents were 54.34 ± 2.35%, in which the microalgae-bacteria consortium improves the removal performance of SMX. The main process of SMX removal can be attributed to the symbiotic biodegradation by bacteria due to the increase of oxygen released by the microalgae photosynthetic process. Therefore, the microalgae-bacteria consortium used in this study, demonstrated to be a promising alternative for bioremediation of SMX, with potential for removal others contaminants from wastewater effluent. However, the residual SMX and the relative abundance of antibiotics resistance genes (ARG) found in this study suggest that SMX contributes to selective pressure for ARG maintenance and proliferation inWWTP effluent. Thus, further studies to removal ARG from WWTP effluent are needed.
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    Distribution of genetic elements associated with antibiotic resistance in treated and untreated animal husbandry waste and wastewater.
    (2021) Pereira, Andressa Rezende; Paranhos, Aline Gomes de Oliveira; Aquino, Sergio Francisco de; Silva, Silvana de Queiroz
    Animal breeding for meat production based on swine, cattle, poultry, and aquaculture is an activity that generates several impacts on the environment, among them the spread of antibiotic resistance. There is a worldwide concern related to the massive use of antibiotics, which causes selective pressure on the microbial community, triggering bacteria that contain “antibiotic resistance genes.” According to the survey here presented, antibiotic resistance-related genes such as tetracyclines (tet), erythromycin (erm), and sulfonamides (sul), as well as the genetic mobile element interferon (int), are the most reported genetic elements in qualitative and quantitative studies of swine, cattle, poultry, and aquaculture manure/wastewater. It has been observed that biological treatments based on waste composting and anaerobic digestion are effective in ARG removal, particularly for tet, bla, erm, and qnr (quinolone) genes. On the other hand, sul and intI genes were more persistent in such treatments. Tertiary treatments, such advanced oxidative processes, are suitable strategies to improve ARG reduction. In general temperature, hydraulic retention time, and penetration of sunlight are the main operational parameters for ARG reduction in treatments applied to animal waste, and therefore attention should be addressed to optimize their efficacy regarding ARG removal. Despite being reduced, the presence of ARG in treated effluents and in biosolids indicates that there is a potential risk of antibiotic resistance spread in nature, especially through the release of treated livestock waste into the environment.
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    Remoção de carbendazim de água por processo de clarificação acoplado à adsorção ou cloração.
    (2018) Pereira, Andressa Rezende; Aquino, Sergio Francisco de; Aquino, Sergio Francisco de; Brandt, Emanuel Manfred Freire; Santiago, Aníbal da Fonseca
    Apesar do carbendazim, fungicida da classe dos benzimidazóis, ser um dos 10 ingredientes ativos de pesticidas mais utilizados nas lavouras brasileiras, pouco se sabe a respeito da sua remoção pelos processos comumente utilizados nas Estações de Tratamento de Águas (ETAs). Assim sendo, o objetivo deste trabalho é avaliar a remoção do carbendazim nas etapas de clarificação acoplada à adsorção e cloração em escala de bancada. Todos os ensaios foram feitos com dois tipos de água, de alta (~200 NTU) e baixa turbidez (~20 NTU), fixando-se a concentração inicial do agrotóxico em 250 μg.L-1. Para a clarificação, foram testados os coagulantes sulfato de alumínio, cloreto de polialumínio e cloreto férrico em doses e pH otimizados previamente para a remoção de partículas suspensas da água. A adsorção com carvão ativado em pó (CAP) foi testada dosando-se o CAP (2,5; 5 e 10 mg.L-1) juntamente com o coagulante ou após a clarificação. Já a cloração foi testada após a clarificação utilizando tempo de contato de até 30 min, sendo o hipoclorito de sódio o agente oxidante na dose inicial de Cl2 igual a 2 mg.L-1. A análise de carbendazim nas amostras de água foi feita por cromatografia líquida acoplada à espectrometria de massas (HPLC-MS-IT-TOF) após validação do método analítico, que resultou em limites de detecção e quantificação de, respectivamente, 1,05 μg.L-1 e 3,49 μg.L-1. A etapa de clarificação resultou em remoções variadas do agrotóxico, sendo o cloreto férrico mais eficiente para as águas de alta turbidez (67%) e o cloreto de polialumínio para águas de baixa turbidez (86%). Menor remoção de carbendazim foi observada na clarificação de águas de maior turbidez, possivelmente porque as partículas suspensas recobrem o hidróxido metálico formado (de carga positiva) diminuindo a área de contato e a interação com o carbendazim (de carga líquida negativa). A adição de CAP junto com o coagulante e como pós-tratamento não resultou em melhoria considerável da clarificação para pequenas doses de CAP (2,5 e 5 mg.L-1), sendo verificada uma eficiência de remoção mais acentuada apenas na maior dose de CAP (10 mg.L-1). Da mesma forma que na clarificação, a presença de partículas suspensas na água influenciou negativamente a adsorção do carbendazim, possivelmente devido ao recobrimento do adsorvente e redução de área e sítios de adsorção. A cloração acoplada à clarificação possibilitou elevada remoção de carbendazim, resultando em concentrações finais inferiores ao limite de quantificação do método. Porém, foi possível perceber a formação e acúmulo de subprodutos clorados do carbendazim, fazendo-se necessário um estudo mais detalhado para elucidação estrutural destes compostos e verificação de sua toxicidade.
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    Remoção de Carbendazim em águas de abastecimento por clarificação acoplada à adsorção em escala de bancada.
    (2021) Pereira, Andressa Rezende; Castro, Paulo Bernardo Neves e; Afonso, Robson José de Cássia Franco; Aquino, Sergio Francisco de
    O objetivo deste artigo é avaliar a remoção do fungicida Carbendazim da água durante a clarificação acopla- da ou não à adsorção em carvão ativado em pó (CAP) em escala de bancada. Para a clarificação, utilizaram-se os coagulantes sulfato de alumínio, o cloreto de polialumínio e o cloreto férrico em dose e pH otimizados previamente para remoção de sólidos em suspensão de águas de baixa (20 NTU) ou alta (200 NTU) turbidez. A adsorção com CAP (2,5; 5 e 10 mg L-1) foi testada adicionando-o com o coagulante ou após a clarificação. A etapa de clarificação resultou em remoções variadas do agrotóxico, sendo o cloreto férrico mais eficiente para águas de alta turbidez (67%) e o cloreto de polialumínio para água de baixa turbidez (86%). A adição de CAP com o coagulante resultou em uma melhoria na eficiência de remoção apenas na dose mais elevada do adsorvente, chegando a 87% de eficiência para a dose de 10 mg L-1 de CAP em águas de baixa turbidez.
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    Removal of cephalexin and erythromycin antibiotics, and their resistance genes, by microalgae-bacteria consortium from wastewater treatment plant secondary effluents.
    (2021) Rodrigues, Daniel Aparecido da Silva; Cunha, Camila Cristina Rodrigues Ferreira da; Espirito Santo, Daiana Rocha do; Barros, André Luis Corrêa de; Pereira, Andressa Rezende; Silva, Silvana de Queiroz; Santiago, Aníbal da Fonseca; Afonso, Robson José de Cássia Franco
    Antibiotics have become a concern in the aquatic environments owing to the potential development of bacterial resistances. Thus, this study evaluated the removal of cephalexin (CEP) and erythromycin (ERY) from a local wastewater treatment plant (WWTP) effluent, mediated by microalgae-bacteria consortium. Likewise, the removal of correlated antibiotics resistance genes blaTEM and ermB was also assessed. The incubation results showed that the added concentrations of selected antibiotics did not restrain the consortium growth. Moreover, CEP and ERY were almost completely removed after the cultivation period, reaching total removals of 96.54% and 92.38%, respectively. The symbiotic interaction between microalgae and bacteria plays a role in the kinetics removal of CEP and ERY. The abundance of blaTEM and ermB was reduced by 0.56 and 1.75 logs, respectively. Lastly, our results suggest that technology based on natural microalgae-bacteria consortium could be a potential alternative to improve the quality of WWTP effluents.
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    Tylosin in anaerobic reactors : degradation kinetics, efects on methane production and on the microbial community.
    (2022) Paranhos, Aline Gomes de Oliveira; Pereira, Andressa Rezende; Fonseca, Yasmim Arantes da; Silva, Silvana de Queiroz; Aquino, Sergio Francisco de
    Tylosin eliminated in animal waste, during therapeutic treatment, can be efciently removed in anaerobic systems. The present study investigated the infuence of tylosin concentration and assessed its degradation kinetics and the microorganisms involved in each stage of its anaerobic digestion (hydrolysis/ acidogenesis; acetogenesis; methanogenesis). The results showed a stimulating efect on methane production with increasing tylosin concentration in the poultry litter up to 80 mg kg−1 tylosin (232.9 NL CH4 kg SV−1). As for tylosin degradation, greater removal of antibiotics was observed in the methanogenic phase (88%), followed by acetogenic (84%) and hydrolytic/acidogenic (76%) phases. The higher rate of tylosin degradation obtained in the methanogenic step, is mainly related to the co-metabolic efect exerted by the presence of acetate and its degradation by acetoclastic methanogens. Indeed, metagenomic analyses suggested a syntrophic action between archaea of the genus Methanobacterium, and bacteria such as Clostridium and Flexilinea, which seemed decisive for tylosin degradation.