Browsing by Author "Rodrigues, Ivanildes Vasconcelos"
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Item Antibacterial activity of extract and fractions from branches of Protium spruceanum and cytotoxicity on fibroblasts.(2017) Amparo, Tatiane Roquete; Rodrigues, Ivanildes Vasconcelos; Seibert, Janaína Brandão; Souza, Rafaella Hilda Zaniti; Oliveira, Amanda Ribeiro de; Cabral, Vivette Appolinário Rodrigues; Vieira, Paula Melo de Abreu; Brandão, Geraldo Célio; Okuma, Adriana Akemi; Vieira Filho, Sidney Augusto; Teixeira, Luiz Fernando de Medeiros; Souza, Gustavo Henrique Bianco deThe crude ethanol extract (CEE) and fractions from branches of Protium spruceanum were subjected to antibacterial and cytotoxicity assays. Compounds of the most active fraction were identified by GC-MS and LC-MS. CEE was active against 19 bacteria and the ethyl acetate fraction (EAF) showed the lowest minimum bactericidal concentration (MBC 0.3–80.0 mg/mL). Through time-kill assay was observed that EAF induced rapid bactericidal effect against Staphylococcus saprophyticus. The cytotoxicity tests against L929 fibroblasts showed great potential of EAF on the treatment of infections caused by five bacteria (MBC < IC50). The results provide in vitro scientific support to the possible application of branches of P. spruceanum as antimicrobial agent that may contribute for treatment of infections.Item Antibacterial substances from leaves of Protium spruceanum (Burseraceae) : in vitro and in silico evaluation.(2020) Amparo, Tatiane Roquete; Rodrigues, Ivanildes Vasconcelos; Seibert, Janaína Brandão; Almeida, Tamires Cunha; Cabral, Vivette Appolinário Rodrigues; Vieira, Paula Melo de Abreu; Brandão, Geraldo Célio; Oliveira, Mauro Lúcio Gonçalves de; Silva, Glenda Nicioli da; Santos, Orlando David Henrique dos; Vieira Filho, Sidney Augusto; Teixeira, Luiz Fernando de Medeiros; Souza, Gustavo Henrique Bianco deDue to the increase of bacterial resistance, the search for new antibiotics is necessary and the medicinal plants represent its most important source. The aim of this study was to evaluate the antibacterial property of extract and fractions from Protium spruceanum leaves, against pathogenic bacteria. By means of diffusion and microdilution assays, the crude extract was active against the nine bacteria tested being the hydromethanolic fraction the most active. During phytochemical procedures, procyanidin (1) and catechin (2) were identified as the main antibacterial constituents of this fraction. In silico results obtained using PASSonline tool indicated 1 and 2 as having good potential to interact with different targets of currently used antibiotics. These results no indicated potential to none DNA effect and indicated the cell wall as mainly target. Electrophoresis result supported that had no DNA damage. Cell wall damage was confirmed by propidium iodide test that showed increased membrane permeability and by cell surface deformations observed in scanning electronic microscopy. The in vitro assays together with the in silico prediction results establish the potential of P. spruceanum as source of antibacterial compounds that acts on important bacterial targets. These results contribute to the development of natural substances against pathogenic bacteria and to discovery of new antibiotics.Item Antiedematogenic and antinociceptive effects of leaves extracts from Protium spruceanum Benth.(2013) Rodrigues, Ivanildes Vasconcelos; Souza, Juliana Neves de Paula; Silva, Ana Cláudia G.; Chibli, Lucas Apolinário; Cabral, Vivette Appolinário Rodrigues; Vieira Filho, Sidney Augusto; Perazzo, Fábio Ferreira; Guimarães, Andrea Grabe; Souza, Gustavo Henrique Bianco deEthnopharmacological relevance: In traditional medicine, gums and oil-resins from Protium species have been used for many diseases, however, there are no reports of studies of Protium spruceanum’ leaves. Materials and methods: The antiedematogenic and antinociceptive effects of crude ethanol extract (EEB) from leaves of P. spruceanum and its fractions were evaluated in biological models. The fractions were obtained with hexane (FHEX) and methanol (FMEOH). Rat paw edema induced by carrageenan, writhing test, formalin test, hot plate test and the toxicity of EEB were performed. Results: Phytochemical analysis has shown the presence of a and b-amyrins as major constituents of FHEX. Promising results of anti-inflammatory and antinociceptive activity were found for EEB, FHEX and FMEOH. Also were observed the EEB at 6000 mg/kg showed no toxicity. Conclusions: One might suggest that the activities of FHEX are due to the presence of a and b-amyrins and contributes to the biological activities of EEB.Item Antitumor effect of Cymbopogon densiflorus (Linneu) essential oil in bladder cancer cells.(2020) Pereira, Gizele Lucia da Costa; Almeida, Tamires Cunha; Seibert, Janaína Brandão; Amparo, Tatiane Roquete; Soares, Rodrigo Dian de Oliveira Aguiar; Rodrigues, Ivanildes Vasconcelos; Souza, Gustavo Henrique Bianco de; Santos, Orlando David Henrique dos; Silva, Glenda Nicioli daThe aim of this study was to analyse the antitumor effect of the Cymbopogon densiflorus essential oil in silico and in vitro on bladder cancer cells RT4 and T24, with different TP53 status. The oil was extracted by hydrodistillation and the gas chromatography coupled to the mass spectrometry was used for characterisation. In silico analysis was carried out by Pass online software. Cytotoxicity, cell proliferation, cell cycle progression, apoptosis and wound healing assays were performed. Five major compounds were identified. In silico analysis showed that major compounds present high potential for antitumor activities. The treatment with C. densiflorus essential oil reduced cell viability of bladder cancer cells. Only in wild-type cells, the increase of apoptosis rates and the decrease of cell migration were observed. In conclusion, the C. densiflorus essential oil presents antitumor effects on TP53 wild-type and mutated bladder cancer cells, however, the mechanism of action is TP53 status-dependent.Item Bioprospecção das atividades antioxidante e antimicrobiana de espécies vegetais medicinais coletadas em Ouro Preto-MG.(2013) Souza, Juliana Neves de Paula; Candotti, Julia Gesualdi; Amparo, Tatiane Roquete; Coelho, Fabiana Fioravante; Rodrigues, Ivanildes Vasconcelos; Santos, Orlando David Henrique dos; Medeiros, Luiz Fernando Teixeira de; Furtado, Niege Araçari Jacometti Cardoso; Sousa, Hildeberto Caldas de; Souza, Gustavo Henrique Bianco deO presente estudo foi realizado com os extratos etanólicos brutos de seis espécies vegetais (Bathysaaustralis, Piper corcovadensis, Siparuna brasiliensis, Picramnia sp., Piper richardiifolium, Eugenia cf. cerasiflora) de uso medicinal, coletadas na região de Ouro Preto-MG. Os extratos etanólicos foram avaliados frente à atividade antioxidante (método do DPPH) e antimicrobiana (Concentração Inibitória Mínima, CIM), bem como submetidos à dosagem de compostos fenólicos (reagente Folin-Ciocalteau) e flavonóides totais (Cloreto de Alumínio). Os resultados evidenciaram que o extrato de Siparuma brasiliensis apresentou maior atividade antioxidante (CE50 17,712 μg/mL) e o de Piper richardiifolium a melhor atividade antimicrobiana para Staphylococcus aureus (300 μg/mL) e Staphylococcus saprophyticus (200 μg/mL). Estes resultados sugerem que as atividades avaliadas estão diretamente relacionadas com a presença de compostos fenólicos, porém não necessariamente à presença de flavonoides.Item Estudo biomonitorado para avaliação das atividades antiinflamatória e analgésica da folhas da espécie Protium spruceanum (Benth) Engler. (Burseraceae).(Programa de Pós-Graduação em Ciências Farmacêuticas. CIPHARMA, Escola de Farmácia, Universidade Federal de Ouro Preto., 2010) Rodrigues, Ivanildes Vasconcelos; Souza, Gustavo Henrique Bianco deNesta dissertação foi realizado o estudo biomonitorado das atividades analgésica e antiinflamatória in vivo e da propriedade antioxidante in vitro do extrato etanólico bruto (EEB) das folhas de Protium spruceanum (Benth.) Engler. Foram utilizados ensaios de detecção das principais classes de metabólitos secundários presentes no EEB e técnicas cromatográficas para isolamento de constituintes majoritários das frações mais ativas. Os teores de FT e de FVT foram determinados por métodos espectrométricos. As atividades farmacológicas in vivo foram avaliadas pelo método de edema de pata induzido por carragenina para determinar a atividade antiinflamatória e métodos de contorções abdominais induzidas por ácido acético, placa quente e formalina para determinação da atividade antinociceptiva. A toxicidade aguda foi determinada a fim de avaliar os efeitos tóxicos do EEB. Observou-se a presença de compostos fenólicos, taninos, flavonóides, saponinas, triterpenos, esteróides, xantonas e cumarinas. O constituinte majoritário encontrado na fração FHEX foi a mistura de α e β-amirinas. Nas frações FDCM e FMEOHF foram isolados três flavonóides, ainda em fase de identificação. Na determinação da propriedade antioxidante foram observados resultados significativos para EEB e frações FMEOH-1, FDCM e PDCM comparadas ao padrão de ácido gálico, onde FDCM apresentou melhor resultado. O EEB apresentou aproximadamente 18 % de compostos fenólicos e a fração FDCM apresentou maior teor de FVT. Na avaliação da atividade antiinflamatória foi possível observar atividade significativa para FHEX e FMEOH-1. Após o fracionamento de FMEOH-1, as frações FMEOH-F e FDCM apresentaram atividade significativa. Observouse também significativa atividade antinociceptiva para as frações FHEX e FMEOH-1 frente ao método de contorções abdominais. Pelo método de placa quente, o EEB, na maior dose administrada, foi capaz de aumentar o TL inicial após 60 minutos da administração, e após a partição, apenas a FHEX apresentou atividade antinociceptiva significativa. No modelo de formalina, o EEB e a FHEX apresentaram atividade antinociceptiva apenas na Fase II. As atividades analgésica central e periférica, e antiinflamatória da FHEX podem ser atribuídas a presença da mistura dos triterpenos α e β-amirina, principal constituinte desta fração. A atividade antiinflamatória das frações FMEOH-1, FMEOH-F e FDCM pode ser atribuída ao considerável teor de compostos fenólicos, principalmente flavonóides, nestas frações. Não foi possível estabelecer a DL50 em camundongos para a administração do EEB até a dose de 6 g/kg.Item Flavonoids, antioxidant, and antiproliferative activities of Stevia urticifolia.(2017) Machado, Kamilla Nunes; Hernández Tasco, Alvaro José; Salvador, Marcos José; Rodrigues, Ivanildes Vasconcelos; Pessoa, Claudia do Ó; Sousa, Ian Jhemes Oliveira; Ferreira, Paulo Michel Pinheiro; Nascimento, Andréa Mendes doItem Melaleuca leucadendra essential oil promotes loss of cell membrane and wall integrity and inhibits bacterial growth : an in silico and in vitro approach.(2020) Silva, Jessica Paola Bautista; Seibert, Janaína Brandão; Amparo, Tatiane Roquete; Rodrigues, Ivanildes Vasconcelos; Teixeira, Luiz Fernando de Medeiros; Souza, Gustavo Henrique Bianco de; Santos, Orlando David Henrique dosEssential oils are potential antimicrobial agents and can be used as active ingredients in the pharmaceutical, food and cosmetics industries. This work intends to evaluate the antibacterial activity and design a strategy for the proposition of the mechanism of action of Melaleuca leucadendra essential oil. Optimum concentration of the bacteria and the phase where they had the highest pathogenic activity were determined. Results show that for each microorganism it is necessary to use a diferent concentration at the time of adjusting the initial inoculum, and that the time to achieve exponential growth phase varies from one to the other. M. leucadendra essential oil demonstrated in vitro antimicrobial properties. This oil was chemically characterized and the main compounds were evaluated by their mechanism of antibacterial action based on structure–activity analysis. The mechanism is related to the increase of bacteria cell membrane permeability. This indication was confrmed by fow cytometry and transmission electronic microscopy. Thus, in silico analysis is an important tool in the search for new antimicrobial agents and these results showed that M. leucadendra essential oil may be useful on the development of new chemotherapies or food preservation systems.Item Nanostructured systems improve the antimicrobial potential of the essential oil from cymbopogon densiflorus leaves.(2019) Seibert, Janaína Brandão; Rosa, Juliana dos Santos; Almeida, Tamires Cunha; Amparo, Tatiane Roquete; Rodrigues, Ivanildes Vasconcelos; Lanza, Juliane Sousa; Frezard, Frederic Jean Georges; Soares, Rodrigo Dian de Oliveira Aguiar; Teixeira, Luiz Fernando de Medeiros; Souza, Gustavo Henrique Bianco de; Vieira, Paula Melo de Abreu; Barichello, José Mario; Santos, Orlando David Henrique dosThe physicochemical characteristics of nanostructured suspensions are important prerequisites for the success of new drug development. This work aimed to develop nanometric systems containing Cymbopogon densiflorus leaf essential oil and to evaluate their antimicrobial activity. The essential oil was isolated by hydrodistillation from leaves and analyzed by GC-MS. The main constituents were found to be trans-p-mentha-2,8-dien-1-ol, cis-p-mentha2,8-dien-1-ol, trans-p-mentha-1(7),8-dien-2-ol, cis-piperitol, and cis-p-mentha-1(7),8-dien-2-ol. In silico prediction analysis suggested that this oil possesses antimicrobial potential and the main mechanism of action might be the peptidoglycan glycosyltransferase inhibition. Nanoemulsions were prepared by the phase inversion method, and liposomes were made by the film hydration method. Qualitative evaluation of the antimicrobial activity was performed by the diffusion disk assay with 24 microorganisms; all of them were found to be sensitive to the essential oil. Subsequently, this property was quantified by the serial microdilution technique, where the nanoformulations demonstrated improved activity in comparison with the free oil. Bactericidal action was tested by the propidium iodide method, which revealed that free essential oil and nanoemulsion increased cytoplasmic membrane permeability, while no difference was observed between negative control and liposome. These results were confirmed by images obtained using transmission electron microscopy. This study has shown an optimization in the antimicrobial activity of C. densiflorus essential oil by a nanoemulsion and a liposomal formulation of the active substances.Item Protective effect of Baccharis trimera extract on acute hepatic injury in a model of inflammation induced by acetaminophen.(2014) Pádua, Bruno da Cruz; Rossoni Júnior, Joamyr Victor; Magalhães, Cíntia Lopes de Brito; Chaves, Míriam Martins; Silva, Marcelo Eustáquio; Pedrosa, Maria Lúcia; Souza, Gustavo Henrique Bianco de; Brandão, Geraldo Célio; Rodrigues, Ivanildes Vasconcelos; Lima, Wanderson Geraldo de; Costa, Daniela CaldeiraBackground. Acetaminophen (APAP) is a commonly used analgesic and antipyretic. When administered in high doses, APAP is a clinical problem in the US and Europe, often resulting in severe liver injury and potentially acute liver failure. Studies have demonstrated that antioxidants and anti-inflammatory agents effectively protect against the acute hepatotoxicity induced by APAP overdose. Methods.The present study attempted to investigate the protective effect of B. trimera against APAP-induced hepatic damage in rats. The liver-function markers ALT and AST, biomarkers of oxidative stress, antioxidant parameters, and histopathological changes were examined. Results.The pretreatment with B. trimera attenuated serum activities of ALT and AST that were enhanced by administration of APAP. Furthermore, pretreatment with the extract decreases the activity of the enzyme SOD and increases the activity of catalase and the concentration of total glutathione. Histopathological analysis confirmed the alleviation of liver damage and reduced lesions caused by APAP. Conclusions.The hepatoprotective action of B. trimera extractmay rely on its effect on reducing the oxidative stress caused by APAP-induced hepatic damage in a rat model. General Significance. These results make the extract of B. trimera a potential candidate drug capable of protecting the liver against damage caused by APAP overdose.Item Seasonality study of essential oil from leaves of Cymbopogon densiflorus and nanoemulsion development with antioxidant activity.(2019) Seibert, Janaína Brandão; Rodrigues, Ivanildes Vasconcelos; Carneiro, Simone Pinto; Amparo, Tatiane Roquete; Lanza, Juliane Sousa; Frezard, Frederic Jean Georges; Souza, Gustavo Henrique Bianco de; Santos, Orlando David Henrique dosThe development of formulations that maintain the biological and physical chemistry properties of essential oils is an important choice when they are used as an active ingredient. This study aimed to characterize the essential oil from leaves of Cymbopogon densiflorus and evaluate the antioxidant activity of the oil, and to produce a nanoemulsion formulation containing it. The essential oil was obtained by hydrodistillation, and seasonality was analysed every 2 months by gas chromatography–mass spectrometry, showing that more than 90% of the composition was maintained for the whole period and that the major compounds were trans‐p‐menta‐2,8‐dien‐1‐ol, cis‐p‐menta‐2,8‐dien‐1‐ol, trans‐p‐menta‐1(7),8‐dien‐2‐ol, cis‐piperitol, and cis‐p‐menta‐1(7),8‐dien‐2‐ol. Stable nanoemulsions were prepared by phase inversion method encapsulating the essential oil. The antioxidant activity was evaluated by 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical scavenging and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid (ABTS) methods. In the first test, free and nanoemulsified essential oil showed half‐maximal inhibitory concentration (IC50) values equivalent to 14.689 and 3.692 mg mL−1, respectively. In the second test, these values were 0.567 and 0.43 mg mL−1. The development of nanoemulsion‐based essential oil from leaves of C. densiflorus was viable, and the formulated oil was able to reproduce the antioxidant activity at a concentration four times lower than that of the pure essential oil.