Activity of alkaloids from Aspidosperma nitidum against Leishmania (Leishmania) amazonensis.

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dc.contributor.authorVeiga, Andreza do Socorro Silva da
dc.contributor.authorSilveira, Fernando Tobias
dc.contributor.authorSilva, Edilene Oliveira da
dc.contributor.authorDiniz Júnior, José Antônio Picanço
dc.contributor.authorAraújo, Sanderson Corrêa
dc.contributor.authorCampos, Marliane Batista
dc.contributor.authorMarinho, Andrey Moacir do Rosário
dc.contributor.authorBrandão, Geraldo Célio
dc.contributor.authorVale, Valdicley Vieira
dc.contributor.authorPercário, Sandro
dc.contributor.authorDolabela, Maria Fâni
dc.date.accessioned2022-11-04T17:17:07Z
dc.date.available2022-11-04T17:17:07Z
dc.date.issued2022pt_BR
dc.description.abstractThis study evaluated the morphological changes caused by fractions and subfractions, obtained from barks of Aspidosperna nitidum, against L. (L.) amazonensis promastigotes. The ethanolic extract (EE) obtained through the maceration of trunk barks was subjected to an acid–base partition, resulting the neutral (FN) and the alkaloid (FA) fractions, and fractionation under refux, yielded hexane (FrHEX), dichloromethane (FrDCL), ethyl acetate (FrACoET), and methanol (FrMEOH) fractions. The FA was fractionated and three subfractions (SF5-6, SF8, and SF9) were obtained and analyzed by HPLC–DAD and 1 H NMR. The antipromastigote activity of all samples was evaluated by MTT, after that, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for the active fractions were performed. Chromatographic analyzes suggest the presence of alkaloids in EE, FN, FA, and FrDCL. The fractionation of FA led to the isolation of the indole alkaloid dihydrocorynantheol (SF8 fractions). The SF5-6, dihydrocorynantheol and SF-9 samples were active against promastigotes, while FrDCL was moderately active. The SEM analysis revealed cell rounding and changes in the fagellum of the parasites. In the TEM analysis, the treated promastigotes showed changes in fagellar pocket and kinetoplast, and presence of lipid inclusions. These results suggest that alkaloids isolated from A. nitidum are promising as leishmanicidal.pt_BR
dc.identifier.citationVEIGA, A. do S. S. da et al. Activity of alkaloids from Aspidosperma nitidum against Leishmania (Leishmania) amazonensis. Scientifc Reports, v. 12, 2022. Disponível em: <https://www.nature.com/articles/s41598-022-12396-x>. Acesso em: 11 out. 2022.pt_BR
dc.identifier.doihttps://doi.org/10.1038/s41598-022-12396-xpt_BR
dc.identifier.issn2045-2322
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/15738
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Fonte: o PDF do artigo.pt_BR
dc.titleActivity of alkaloids from Aspidosperma nitidum against Leishmania (Leishmania) amazonensis.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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