Deep sequencing of small RNAs reveals the repertoire of miRNAs and piRNAs in Biomphalaria glabrata.

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dc.contributor.authorQueiroz, Fábio Ribeiro
dc.contributor.authorPortilho, Laysa Gomes
dc.contributor.authorJeremias, Wander de Jesus
dc.contributor.authorBabá, Élio Hideo
dc.contributor.authorAmaral, Laurence Rodrigues do
dc.contributor.authorSilva, Luciana Maria
dc.contributor.authorCoelho, Paulo Marcos Zech
dc.contributor.authorCaldeira, Roberta Lima
dc.contributor.authorGomes, Matheus de Souza
dc.date.accessioned2022-12-07T20:52:20Z
dc.date.available2022-12-07T20:52:20Z
dc.date.issued2020pt_BR
dc.description.abstractBACKGROUND Biomphalaria glabrata snails are widely distributed in schistosomiasis endemic areas like America and Caribe, displaying high susceptibility to infection by Schistosoma mansoni. After the availability of B. glabrata genome and transcriptome data, studies focusing on genetic markers and small non-coding RNAs have become more relevant. The small RNAs have been considered important through their ability to finely regulate the gene expression in several organisms, thus controlling the functions like cell growth, metabolism, and susceptibility/resistance to infection. OBJECTIVE The present study aims on identification and characterisation of the repertoire of small non-coding RNAs in B. glabrata (Bgl-small RNAs). METHODS By using small RNA sequencing, bioinformatics tools and quantitative reverse transcription polymerase chain reaction (RT-qPCR), we identified, characterised, and validated the presence of small RNAs in B. glabrata. FINDINGS 89 mature miRNAs were identified and five of them were classified as Mollusk-specific. When compared to model organisms, sequences of B. glabrata miRNAs showed a high degree of conservation. In addition, several target genes were predicted for all the mature miRNAs identified. Furthermore, piRNAs were identified in the genome of B. glabrata for the first time. The B. glabrata piRNAs showed strong conservation of uridine as first nucleotide at 5’ end, besides adenine at 10th position. Our results showed that B. glabrata has diverse repertoire of circulating ncRNAs, several which might be involved in mollusk susceptibility to infection, due to their potential roles in the regulation of S. mansoni development. MAIN CONCLUSIONS Further studies are necessary in order to confirm the role of the Bgl-small RNAs in the parasite/host relationship thus opening new perspectives on interference of small RNAs in the organism development and susceptibility to infection.pt_BR
dc.identifier.citationQUEIROZ, F. R. et al. Deep sequencing of small RNAs reveals the repertoire of miRNAs and piRNAs in Biomphalaria glabrata. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 115, 2020. Disponível em: <https://www.scielo.br/j/mioc/a/7nCcmXqFmscfBkRtHXfxhsd/?lang=en>. Acesso em: 11 out. 2022.pt_BR
dc.identifier.doihttps://doi.org/10.1590/0074-02760190498pt_BR
dc.identifier.issn1678-8060
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/15889
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Fonte: o PDF do artigo.pt_BR
dc.subjectSchistosomiasispt_BR
dc.subjectNon-coding RNAspt_BR
dc.subjectMollusk vectorspt_BR
dc.subjectNext-generation sequencingpt_BR
dc.titleDeep sequencing of small RNAs reveals the repertoire of miRNAs and piRNAs in Biomphalaria glabrata.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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