Extraction and fractionation effects on antiplasmodial activity and phytochemical composition of Palicourea hoffmannseggiana

Simple item page
dc.contributor.authorOhashi, Leticia Hiromi
dc.contributor.authorGontijo, Douglas da Costa
dc.contributor.authorNascimento, Maria Fernanda Alves do
dc.contributor.authorMargalho, Luciano Ferreira
dc.contributor.authorBrandão, Geraldo Célio
dc.contributor.authorOliveira, Alaíde Braga de
dc.date.accessioned2021-12-06T20:14:53Z
dc.date.available2021-12-06T20:14:53Z
dc.date.issued2021pt_BR
dc.description.abstractThe present study on Palicourea hoffmannseggiana, which was collected at Marapanim, state of Pará, Brazil, comprises the preparation of different stem and leaf extracts and fractions. Ethanol, hydroethanol, and water extracts were prepared by several methods and evaluated for in vitro activity against resistant Plasmodium falciparum (W2 strain), disclosing a low parasite growth inhibition effect ( < 50 %). Dereplication by UPLC-DAD-ESI −MS of the leaf ethanol extract showed the presence of two known alkaloids, lyalosidic and strictosidinic acids, along with a sinapoyl ester of lyalosidic acid, with m/z 719.33 [M +H] + , which is possibly a new monoterpene indole alkaloid representative. Sequential liquid-liquid acid-base alkaloid separations from the leaf ethanol extract as well as directly from leaf powder afforded fractions of increased parasite growth inhibition, reaching up to 92.5±0.7%. The most bioactive fractions were shown to contain the β-carboline alkaloids harmane and 4-methyl-β-carboline, along with N-methyl-tryptamine and N-acetyl-tryptamine, while monoterpene indole alkaloids were detected in inactive fractions of these processes. The present results demonstrate that these preliminary fractionation methods can lead to significantly active fractions supporting an adequate scale-up to carrying out the isolation of anti-plasmodial compounds.pt_BR
dc.identifier.citationOHASHI, L. H. et al. Extraction and fractionation effects on antiplasmodial activity and phytochemical composition of Palicourea hoffmannseggiana. Planta Medica International Open, v. 8, p. e34-e42, 2021. Disponível em: <https://www.thieme-connect.com/products/ejournals/abstract/10.1055/a-1375-6456>. Acesso em: 10 jun. 2021.pt_BR
dc.identifier.doihttps://doi.org/10.1055/a-1375-6456pt_BR
dc.identifier.issn2509-9264
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/14097
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons. org/licenses/by-nc-nd/4.0/). Fonte: o PDF do artigo.pt_BR
dc.subjectRubiaceaept_BR
dc.subjectMonoterpene indole alkaloidspt_BR
dc.subjectβ-carboline alkaloidspt_BR
dc.subjectPlasmodium falciparumpt_BR
dc.titleExtraction and fractionation effects on antiplasmodial activity and phytochemical composition of Palicourea hoffmannseggianapt_BR
dc.typeArtigo publicado em periodicopt_BR
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ARTIGO_ExtractionFractionationEffects.pdf
Size:
420.07 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
DEFAR - Artigos publicados em periódicos