Development of propolis nanoemulsion with antioxidant and antimicrobial activity for use as a potential natural preservative.
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Date
2019
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Abstract
Propolis has demonstrated potential use as food preservative but it presents strong and unpleasant flavor that
alters the sensory characteristics foods. A nanoemulsion was proposed to carry the Brazilian propolis extracts for
use as natural food preservative. Antimicrobial and antioxidant activities and chemical constituents of the extracts were investigated. The latter were made by sequential extraction using different solvents (hexane, ethyl
acetate and ethanol). Antimicrobial activity was evaluated by agar diffusion and microdilution methods and
antioxidant activity by DPPH and ABTS assays. Extracts showed antibacterial and antioxidant activity, highlighting the ethanolic which contained artepillin-C, kaempferide, drupanin and p-coumaric acid as main compounds by LC-MS analysis. The nanoemulsion developed by phase inversion method was characterized and
stable under thermal-stress and centrifugation conditions. Biological properties evaluated were effectively
maintained by the formulation. It was concluded that the nanoemulsion can be used as a food preservative,
preventing degradation and masking the propolis off-flavor.
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Keywords
Food preservative, Antibacterial
Citation
SEIBERT, J. B. et al. Development of propolis nanoemulsion with antioxidant and antimicrobial activity for use as a potential natural preservative. Food Chemistry, v. 287, p. 61-67, jul. 2019. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0308814619304017>. Acesso em: 25 fev. 2019.