PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy.

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dc.contributor.authorPereira, Maira Alves
dc.contributor.authorMosqueira, Vanessa Carla Furtado
dc.contributor.authorVilela, José Mário Carneiro
dc.contributor.authorAndrade, Margareth Spangler
dc.contributor.authorRamaldes, Gilson Andrade
dc.contributor.authorCardoso, Valbert Nascimento
dc.date.accessioned2014-11-11T18:17:34Z
dc.date.available2014-11-11T18:17:34Z
dc.date.issued2008
dc.description.abstractThe present work describes the preparation, characterization and labelling of conventional and surface-modified nanocapsules (NC) with 99mTc-HMPAO. The size, size distribution and homogeneity were determined by photon correlation spectroscopy (PCS) and zeta potential by laser doppler anemometry. The morphology and the structural organization were evaluated by atomic force microscopy (AFM). The stability and release profile of the NC were determined in vitro in plasma. The results showed that the use of methylene blue induces significant increase in the encapsulation efficiency of 99mTc-HMPAO, from 24.4 to 49.8% in PLANC and 22.37 to 52.93% in the case of PLA-PEGNC(P < 0.05) by improving the complex stabilization. The average diameter of NC calculated by PCS varied from 216 to 323 nm, while the average diameter determined by AFM varied from 238 to 426 nm. The AFM analysis of diameter/height ratios suggested a greater homogeneity of the surface-modified PLAPEG nanocapsules compared to PLA NC concerning their flattening properties. The in vitro release of the 99mTc-HMPAO in plasma medium was faster for the conventional PLA NC than for the surface-modified NC. For the latter, 60% of the radioactivity remained associated with NC, even after 12h of incubation. The results suggest that the surface-modified 99mTc-HMPAO-PLA PEG NCwas more stable against label leakage in the presence of proteins and could present better performance as radiotracer in vivo.pt_BR
dc.identifier.citationPEREIRA, M. A. et al. PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy. European Journal of Pharmaceutical Sciences. V. 33, p. 42-51, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0928098707003636>. Acesso em: 20 ago. 2014.pt_BR
dc.identifier.issn0928-0987
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/3771
dc.language.isoen_USpt_BR
dc.rights.licenseO periódico Advanced Drug Delivery Reviews concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3460320800963.pt_BR
dc.subjectNanocapsulespt_BR
dc.subjectAtomic force microscopypt_BR
dc.subjectPhysicochemical characterizationpt_BR
dc.subjectPhoton correlation spectroscopypt_BR
dc.subjectRadiotracerpt_BR
dc.titlePLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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