Cell surface analysis of trypanosomes of the subgenus Schizotrypanum isolate from bats.

Pinto, Artur da Silveira; Teixeira, Luiz Fernando de Medeiros; Padron, Thais Cristina Baeta Soares Souto; Andrade, Arnaldo Feitosa Braga de

Cell surface analysis of trypanosomes of the subgenus Schizotrypanum isolate from bats.

Date

1996

Authors

Pinto, Artur da Silveira

Teixeira, Luiz Fernando de Medeiros

Padron, Thais Cristina Baeta Soares Souto

Andrade, Arnaldo Feitosa Braga de

Abstract

Two stocks (M5, M29) of trypanosomes of the subgenus Schizotrypanum were isolated from the bat Phylloslomus hastatus and analyzed for cell electrophoretic mobility (EPM) and lectin binding surface sites. Epimastigotes from the M5 and M29 stocks presented a mean EPM of around - 0.57 and -0.56 μm, s-1.V-1.cm, respectively. Differences in the agglutination profiles were detected between epimastigotes or trypomastigotes from the two parasite populations using lectins with specificity for D-GlcNAc, D-GalNAc, D-Gal and D-Man as probe. Major variation was observed between epimastigote forms. Additionally, the D-GlcNAc binding lectins WGA and BS II strongly interacted with the trypomastigote from both M5 and M29 stocks; this fact is evidence that these trypanosomes are distinct from Trypanosoma (Schizotrypanum) cruzi.

Keywords

Electrophoretic mobility, Lectin agglutination

Citation

PINTO, A. S. et al. Cell surface analysis of trypanosomes of the subgenus Schizotrypanum isolate from bats. Parasite, Paris, v. 3, p. 143-146, 1996. Disponível em: <http://www.parasite-journal.org/articles/parasite/abs/1996/02/parasite1996032p143/parasite1996032p143.html>. Acesso em: 20 jan. 2017.