Cytotoxic potential of 14 Passiflora species against cancer cells.

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dc.contributor.authorAmaral, Ricardo Guimarães
dc.contributor.authorGomes, Silvana Vieira Floresta
dc.contributor.authorLuciano, Maria Claudia dos Santos
dc.contributor.authorPessoa, Claudia do Ó
dc.contributor.authorAndrade, Luciana Nalone
dc.contributor.authorSeverino, Patrícia
dc.contributor.authorBrandão, Geraldo Célio
dc.contributor.authorBomfim, Larissa Mendes
dc.contributor.authorSoares, Milena Botelho Pereira
dc.contributor.authorBezerra, Daniel Pereira
dc.contributor.authorDavid, Jorge Maurício
dc.contributor.authorCarvalho, Adriana Andrade
dc.date.accessioned2020-05-18T16:17:41Z
dc.date.available2020-05-18T16:17:41Z
dc.date.issued2019
dc.description.abstractThis work aimed to evaluate the cytotoxic potential against cancer cells of Passiflora genus plant species cultivated in Brazil and identify the mechanism of cytotoxicity induced by the most promising extract. Ethanolic extracts from the leaves of 14 Passiflora species were obtained by accelerated solvent extraction and in vitro cytotoxicity evaluated against cancer cell lines using MTT assay at a single concentration of 50 μg/ml. Additionally, the IC50 of the Passiflora alata (ELPA) leaf extracts was determined against both cancer (HCT-116, SF-295, OVACAR-8, and HL-60), and non-cancer cells (PBMC). The ELPA flavonoids were identified by HPLC-DAD and UHPLC-MS/MS. The morphological analyses, using light and fluorescence microscopy, and cell cycle and DNA fragmentation analysis, using flow cytometry, were evaluated to study the mechanism of cell death induced by ELPA in HL-60 cells. Among the Passiflora leaf extracts evaluated; ELPA stood out with high cytotoxic activity, followed by Passiflora capsularis and Passiflora quadrangularis with varying high and low cytotoxic activity. ELPA presented high cytotoxic potency in HL-60 (IC50 19.37 μg/ml), and without cytotoxicity against PBMC, suggesting selectivity for cancer cells. The cytotoxic activity of ELPA may well be linked to the presence of ten identified flavonoids. Cells treated with ELPA presented the hallmarks typical of apoptosis and necrosis, with cell cycle arrest in the G2/M phase. From among the studied species, ELPA presented greater cytotoxic activity, possibly a consequence of synergistic flavonoid action, which induces cell death by apoptosis and necrosis.pt_BR
dc.identifier.citationAMARAL, R. G. et al. Cytotoxic potential of 14 Passiflora species against cancer cells. Journal of Medicinal Plant Research, v. 13, n. 7, p. 157-166, abr. 2019. Disponível em: <https://academicjournals.org/journal/JMPR/article-full-text-pdf/118302260767>. Acesso em: 10 fev. 2020.pt_BR
dc.identifier.doihttps://doi.org/10.5897/JMPR2019.6744pt_BR
dc.identifier.issn1996-0875
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/12211
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseAuthor(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License. Fonte: o próprio artigo.pt_BR
dc.subjectEthanolic extractpt_BR
dc.subjectPassiflora alatapt_BR
dc.titleCytotoxic potential of 14 Passiflora species against cancer cells.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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