Purification and substrate specificity of an angiotensin converting elastase-2 from the rat mesenteric arterial bed perfusate.

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dc.contributor.authorPaula, Carmem Aparecida de
dc.contributor.authorSousa, Marcelo Valle de
dc.contributor.authorSalgado, Maria Cristina de Oliveira
dc.contributor.authorOliveira, Eduardo Brandt de
dc.date.accessioned2017-02-23T17:13:49Z
dc.date.available2017-02-23T17:13:49Z
dc.date.issued1998
dc.description.abstractA soluble angiotensin (Ang) II-generating enzyme has been purified to homogeneity from the rat mesenteric arterial bed(MAB) perfusate by a combination of gel filtration and affinity chromatographies. The enzyme is a glycoprotein of 28.5 kDa(SDS-PAGE), whose N-terminal sequence is identical with that of the rat pancreatic elastase-2 ; therefore the enzyme willhenceforth be referred to as rat MAB elastase-2. When Ang I was used as the substrate, the enzyme specifically released AngII and the dipeptide His-Leu (Km=36WM;Kcat= 1530 min31). The catalytic efficiency (Kcat/Km= 42.5 min31WM31) of thisreaction was comparable to those of other known Ang I-converting enzymes. The proteolytic specificity of the purifiedenzyme toward mellitin, oxidized insulin B chain, somatostatin-14 and renin substrate tetradecapeptide suggested that theenzyme-substrate interaction was defined by an extended substrate binding site, typical of elastases-2 of pancreatic origin. According to the sensitivity of the rat MAB elastase-2 to various inhibitors this enzyme could be described as a member ofthe chymostatin-sensitive group of Ang II-forming serine proteases. The localization and biochemical properties of thisenzyme suggest that it might play a role in the regional control of vascular tonus.pt_BR
dc.identifier.citationPAULA, C. A. de et al. Purification and substrate specificity of an angiotensin converting elastase-2 from the rat mesenteric arterial bed perfusate. Biochimica Et Biophysica Acta, v. 1388, p.227-238, 1998. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0167483898001861>. Acesso em: 10 out. 2016.pt_BR
dc.identifier.doihttps://doi.org/10.1016/S0167-4838(98)00186-1
dc.identifier.issn1570-9639
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/7306
dc.identifier.uri2http://www.sciencedirect.com/science/article/pii/S0167483898001861pt_BR
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.subjectChymasept_BR
dc.subjectSomatostatinpt_BR
dc.titlePurification and substrate specificity of an angiotensin converting elastase-2 from the rat mesenteric arterial bed perfusate.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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