Differential expression of iron metabolism proteins in diabetic and diabetic iron-supplemented rat liver.
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Date
2012
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Abstract
Diabetes mellitus is associated with altered
iron homeostasis that can potentially effect reactive
oxygen species generation and contribute to
diabetes-related complications. We investigated, by
quantitative polymerase chain reaction, whether the
expression of liver hepcidin, ferritin, and TfR-1 is altered
in diabetes. Rats in the control (C) group received
a standard diet; control iron (CI) group received a standard
diet supplemented with iron; diabetic (D) group
received an injection of streptozotocin; and diabetic
iron (DI) group received streptozotocin and the diet
with iron.Animals of theDgroup showed higher levels
of serum iron, increased concentration of carbonyl protein,
and a decrease in antioxidant status. Group D rats
showed increased hepatic expression of Trf-1 compared
to the other groups. Iron supplementation reversed this
increase. Hepcidin mRNA was 81% higher in DI than
in C and CI rats. The results suggest that diabetes,
with or without excess iron, can cause perturbations
in iron status, hepcidin and Trf-1 expression.
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Keywords
Carbonyl iron, Streptozotocin, Hepcidin, Ferritin
Citation
SILVA, M. et al. Differential expression of iron metabolism proteins in diabetic and diabetic iron-supplemented rat liver. Journal of Biochemical and Molecular Toxicology, v. 26, p. 123-129, 2012. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1002/jbt.20418/epdf>. Acesso em: 20 de jul. 2017.