Cloxacillin benzathine-loaded polymeric nanocapsules : physicochemical characterization, cell uptake, and intramammary antimicrobial effect.

Simple item page
dc.contributor.authorAraújo, Raquel Silva
dc.contributor.authorGarcia, Giani Martins
dc.contributor.authorVilela, José Mário Carneiro
dc.contributor.authorAndrade, Margareth Spangler
dc.contributor.authorOliveira, Laser Antônio Machado de
dc.contributor.authorKano, Eunice Kazue
dc.contributor.authorLange, Carla Christine
dc.contributor.authorBrito, Maria Aparecida Vasconcelos Paiva
dc.contributor.authorBrandão, Humberto de Mello
dc.contributor.authorMosqueira, Vanessa Carla Furtado
dc.date.accessioned2020-05-26T15:05:11Z
dc.date.available2020-05-26T15:05:11Z
dc.date.issued2019
dc.description.abstractThe present work shows the development and evaluation of the veterinary antibiotic cloxacillin benzathine (CLOXB) loaded into poly-ε-caprolactone (PCL) nanocapsules (NC), as a potential new treatment strategy to manage bovine intramammary infections, such as mastitis. Staphylococcus aureus-induced mastitis is often a recurrent disease due to the persistence of bacteria within infected cells. CLOXB-PCL NC were prepared by interfacial deposition of preformed biodegradable polymer followed by solvent displacement method. The mean diameter of NC varied from 241 to 428 nm and from 326 to 375 nm, when determined by dynamic light scattering and by atomic force microscopy, respectively. The zeta potential of NC was negative and varied from −28 to −51 mV. In vitro release studies from the NC were performed in two media under sink conditions: PBS with 1% polyethylene glycol or milk. A reversed-phase HPLC method was developed to determine the NC entrapment efficiency and kinetics of CLOXB release from the NC. Free CLOXB dissolution occurred very fast in both media, while drug release from the NC was slower and incomplete (below 50%) after 9 h. CLOXB release kinetics from polymeric NC was fitted with the Korsmeyer-Peppas model indicating that CLOXB release is governed by diffusion following Fick's law. The fluorescence confocal microscopy images of macrophage-like J774A.1 cells reveal NC uptake and internalization in vitro. In addition, antimicrobial effect of the intramammary administration of CLOXB-PCL NC in cows with mastitis resulted in no clinical signs of toxicity and allowed complete pathogen elimination after treatment. The in vivo results obtained in this work suggest that CLOXB-PCL NC could be a promising formulation for the treatment of intramammary infections in cattle, considering their physicochemical properties, release profiles and effects on bovine mastitis control.pt_BR
dc.identifier.citationARAÚJO, R. S. et al. Cloxacillin benzathine-loaded polymeric nanocapsules : physicochemical characterization, cell uptake, and intramammary antimicrobial effect. Materials Science & Engineering C-Materials for Biological Applications, v. 104, p. 110006, nov. 2019. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0928493119302267>. Acesso em: 10 fev. 2020.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.msec.2019.110006pt_BR
dc.identifier.issn0928-4931
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/12253
dc.identifier.uri2https://www.sciencedirect.com/science/article/pii/S0928493119302267pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectRelease kineticspt_BR
dc.subjectMastitispt_BR
dc.titleCloxacillin benzathine-loaded polymeric nanocapsules : physicochemical characterization, cell uptake, and intramammary antimicrobial effect.pt_BR
dc.typeArtigo publicado em periodicopt_BR
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ARTIGO_CloxacillinBenzathineLoaded.pdf
Size:
1.5 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
924 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
DEFAR - Artigos publicados em periódicos