High resolution mass spectrometry elucidation of captopril´s ozonation and chlorination by-products.
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Date
2017
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Abstract
The article evaluated the degradation of the captopril in aqueous solution after
ozonation and chlorination. The process was continuously monitored focusing
on the identification, mass spectrometry and elucidation of its by-products
by applying direct infusion and high performance liquid chromatography,
electrospray ionization high resolution mass spectrometry, in the negative
ion mode. The cytotoxicity of its by-products solutions were evaluated
with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT)
assay. It was observed through that after 30 min of ozonation and chlorination,
there was complete oxidation of captopril, i .e ., 100% removal efficiency.
At these conditions, the rate of mineralization, by total organic carbon, was
only 7.63% for ozonation and 6.40% for chlorination, evidencing the formation
of degradation by-products. Ten captopril by-products were identified
and their respective chemical structures elucidations are proposed. The treated
samples and their by-products were nontoxic to HepG2 cells by MTT assay.
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Liquid chromatography
Citation
QUINTÃO, F. J. O. et al. High resolution mass spectrometry elucidation of captopril´s ozonation and chlorination by-products. American Journal Of Analytical Chemistry, v. 08, p. 264-279, 2017. Disponível em: <http://www.scirp.org/journal/PaperInforCitation.aspx?PaperID=75798>. Acesso em: 29 ago. 2017.