Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae.

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dc.contributor.authorAelst, Linda Van
dc.contributor.authorHohmann, Stefan
dc.contributor.authorBulaya, Botchaka
dc.contributor.authorKoning, Wim de
dc.contributor.authorSierkstra, Laurens
dc.contributor.authorNeves, Maria José
dc.contributor.authorLuyten, Kattie
dc.contributor.authorAlijo, Rafael
dc.contributor.authorRamos, José
dc.contributor.authorCoccetti, Paola
dc.contributor.authorMartegani, Enzo
dc.contributor.authorRocha, Neuza Maria de Magalhaes
dc.contributor.authorBrandão, Rogélio Lopes
dc.contributor.authorDijck, Patrick Van
dc.contributor.authorVanhalewyn, Mieke
dc.contributor.authorDurnez, Peter
dc.contributor.authorJans, Arnold W. H
dc.contributor.authorThevelein, Johan Maria
dc.date.accessioned2017-03-22T17:04:17Z
dc.date.available2017-03-22T17:04:17Z
dc.date.issued1993
dc.description.abstractCells of the yeast Saccharomyces cerevisiae display a wide range of glucose-induced regulatory phenomena, including glucose-induced activation of the RAS-adenylate cyclase pathway and phosphatidylinosrtot turnover, rapid post-translatronal effects on the activity of different enzymes as well as long-term effects at the transcriptional level. A gene called GGS1 (for general Glucose Sensor) that is apparently required for the glucose-induced regulatory effects and several ggsi aHeles (fic/pf, bypi and cifi) has been cloned and characterized. A GGS1 homologue is present in Methanobacterium thermoautotrophicum. Yeast ggsi mutants are unable to grow on glucose or Received 25 November, 1992; revised and accepted 15 February, 1993. •For correspondence. Tel. (16) 220931; Fax (16) 204415. tThese two authors contributed equally to this paper. related readily fermentable sugars, apparently owing to unrestricted influx of sugar Into glycolysis, resulting in its rapid deregulation. Levels of intracellular free glucose and metabolites measured over a period of a few minutes after addition of glucose to cells of a ggsi^ strain are consistent with our previous suggestion of a functional interaction between a sugar transporter, a sugar kinase and the GGS1 gene product. Such a glucose-sensing system might both restrict the influx of glucose and activate several signal transduction pathways, leading to the wide range of glucose-induced regulatory phenomena. Deregulation of these pathways in ggsi mutants might explain phenotypic defects observed in the absence of glucose, e.g. the inability of ggsi diploids to sporulate.pt_BR
dc.identifier.citationAELST, L. V et al. Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae. Molecular Microbiology, v. 82, p. 927-943, 1993. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.1993.tb01638.x/epdf>. Acesso em: 10 jan. 2017.pt_BR
dc.identifier.doihttps://doi.org/10.1111/j.1365-2958.1993.tb01638.x
dc.identifier.issn1365-2958
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/7428
dc.identifier.uri2http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.1993.tb01638.x/epdfpt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.titleMolecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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