Compared vectorial transmissibility of pure and mixed clonal genotypes of Trypanosoma cruzi in Triatoma infestans.
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Date
1998
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Abstract
A total of 15 mixtures involving 9 di erent
stocks attributed to the 19/20, 32 and 39 major clonal
genotypes of Trypanosoma cruzi were used to infect
third-instar nymphs of Triatoma infestans via an arti®-
cial feeding device. Three biological parameters were
considered: (1) the percentage of infected insects (%II),
(2) the number of ¯agellates per insect (NFI), and (3) the
percentage of trypomastigotes per insect (%DIF). Ge-
netic characterization by both multilocus enzyme elec-
trophoresis (MLEE) and random ampli®cation of
polymorphic DNA (RAPD) indicated that in almost all
cases (87%), mixtures remained present after completion
of the whole cycle in the insect vector. Two lines of
comparison were performed: (1) pure clonal genotypes
versus corresponding mixed clonal genotypes and (2)
the actual behavior of mixed clonal genotypes versus the
expected behavior of the theoretical mixture (i.e.
the arithmetic mean of the results observed for each of
the two clonal genotypes taken separately). Statistical
analyses of the variables were made di cult because of
the presence of large standard deviations. Nevertheless,
in several cases, mixtures di ered signi®cantly from pure
clonal genotypes, and in one case the actual mixture
di ered signi®cantly from the theoretical mixture. In
some cases, interaction (either potentialization or re-
ciprocal inhibition) could be suspected.
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PINTO, A. S. et al. Compared vectorial transmissibility of pure and mixed clonal genotypes of Trypanosoma cruzi in Triatoma infestans. Parasitology Research, v. 84, p. 348-353, 1998. Disponível em: <http://link.springer.com/article/10.1007/s004360050409>. Acesso em: 20 jan. 2017.