Morphometric evaluation and nonclassical criteria for the diagnosis of HPV infection and cytological atypia in cervical samples.

Sana, Dandara Emery Morais; Miranda, Priscila Mayrink de; Pitol, Bruna Caroline Vieira; Silva, Nayara Nascimento Toledo; Silva, Ismael Dale Cotrim Guerreiro da; Stocco, Rita de Cássia; Beçak, Willy; Lima, Angélica Alves; Carneiro, Cláudia Martins

Morphometric evaluation and nonclassical criteria for the diagnosis of HPV infection and cytological atypia in cervical samples.

Date

2013

Authors

Sana, Dandara Emery Morais

Miranda, Priscila Mayrink de

Pitol, Bruna Caroline Vieira

Silva, Nayara Nascimento Toledo

Silva, Ismael Dale Cotrim Guerreiro da

Stocco, Rita de Cássia

Beçak, Willy

Lima, Angélica Alves

Carneiro, Cláudia Martins

Abstract

Herein, we evaluated cervical samples from normal tissue or HPV-infected tissue, to determine if the relative nuclear/cytoplasmic ratio (NA/CA) and the presence of nonclassical cytological criteria are a novel cytological criterion for the diagnosis of HPV. Significantly, larger NA/CA ratios were found for the HPV−ATYPIA+ and HPV+ATYPIA+ groups compared with HPV−ATYPIA− group, regardless of collection method. For the samples collected with a spatula, only three samples from the HPV−ATIPIA− group showed four or more nonclassical parameters (i.e., were positive), while a larger number of the samples in the HPV−ATYPIA+, HPV+ATYPIA−, and HPV+ATYPIA+ groups were positive (13, 4, and 13 samples, respectively). Among those collected with a brush, no sample showed four or more nonclassical criteria in the HPV−ATYPIA− group, while a number of samples were positive in the HPV−ATYPIA+, HPV+ATYPIA−, and HPV+ATYPIA+ groups (4, 3, and 4 samples, respectively). HPV infection was associated with significant morphometrical changes; no increase in the NA/CA ratio was found in the HPV+ATYPIA- samples, compared with the HPV−ATIPIA− samples collected with either a spatula or a brush. In conclusion, by including nonclassical cytological criteria into the patient diagnosis, we were able to reduce the number of false negative and false positive HPV diagnoses made using conventional cytology alone. Diagn. Cytopathol.

Keywords

Cervix-vaginal cytology, Morphometry, Molecular biology

Citation

SANA, D. E. M. et al. Morphometric evaluation and nonclassical criteria for the diagnosis of HPV infection and cytological atypia in cervical samples. Diagnostic Cytopathology, v. 41, n. 9, p.785-792, 2013. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1002/dc.22955/abstract;jsessionid=F9558148044C5AD060CD7291C46D88E8.f04t04>. Acesso em: 10 jan. 2017.