Prenylated flavonoid-enriched fraction from maclura tinctoria shows biological activity against Staphylococcus aureus and protects galleria mellonella larvae from bacterial infection.

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dc.contributor.authorAlmeida, Ayla das Chagas
dc.contributor.authorRodrigues, Laís Azevedo
dc.contributor.authorPaulino, Graziela dos Santos
dc.contributor.authorAguilar, Ananda Pereira
dc.contributor.authorAlmeida, Alisson Andrade
dc.contributor.authorFerreira, Sukarno Olavo
dc.contributor.authorBrandão, Geraldo Célio
dc.contributor.authorLeite, João Paulo Viana
dc.contributor.authorRibon, Andrea de Oliveira Barros
dc.date.accessioned2020-05-15T15:47:00Z
dc.date.available2020-05-15T15:47:00Z
dc.date.issued2019
dc.description.abstractBackground: The Atlantic Forest biome extends along the entire Brazilian coast and is home to approximately 20,000 plant species, many of which are endemic; it is considered one of the hotspot regions of the planet. Several of these species are sources of natural products with biological activities that are still unknown. In this study, we evaluated the antimicrobial activity of 90 extracts derived from native Atlantic Forest tree species against Staphylococcus aureus, an important human and veterinary pathogen. Methods: Extracts from native Atlantic Forest tree species were evaluated for their antimicrobial activity against S. aureus by in vitro standard methods. Phytochemical fractionation of the extract from Maclura tinctoria was performed by liquid-liquid partitioning. LC-DAD-ESI-MS was used for identification of constituents in the most active fraction. Damage of cells and alterations in the permeability of cell membrane were determined by atomic force microscopy (AFM) and crystal violet uptake assay, respectively. In vivo antimicrobial activity was evaluated using Galleria mellonella larvae infected with S. aureus with survival data collected using the Kaplan-Meier method. Results: Among the organic or aqueous extracts tested here, 26 showed biological activity. Eight species showed relevant results, with a minimum inhibitory concentration (MIC) below 1 mg/mL. Antibacterial activity was registered for three species for the first time. An organic extract from Maclura tinctoria leaves showed the lowest MIC (0.08 mg/mL). Fractionation of this extract by liquid-liquid partitioning led to obtaining fraction 11FO d with a MIC of 0.04 mg/mL. This fraction showed strong activity against veterinary S. aureus isolates and contributed to the increased survival of Galleria mellonella larvae infected with S. aureus ATCC 29213. The bacterial surface was not altered by the presence of 11FO d, and no cell membrane damage was detected. The LC-DAD-ESI/MS analyses identified prenylated flavonoids as the major constituents responsible for the antibacterial activity of this active extract. Conclusion: A fraction enriched in prenylated isoflavones and flavanones from M. tinctoria showed in vitro and in vivo efficacy as antistaphylococcal agents. These findings justify the need for further research to elucidate the mechanisms of action of these compounds.pt_BR
dc.identifier.citationALMEIDA, A. C. et al. Prenylated flavonoid-enriched fraction from maclura tinctoria shows biological activity against Staphylococcus aureus and protects galleria mellonella larvae from bacterial infection. BMC Complementary and Alternative Medicine, v. 19, n. 189, jul. 2019. Disponível em: <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664575/>. Acesso em: 10 fev. 2020.pt_BR
dc.identifier.doihttps://doi.org/10.1186/s12906-019-2600-ypt_BR
dc.identifier.issn2662-7671
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/12200
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Fonte: o próprio artigo.pt_BR
dc.subjectAntibacterialpt_BR
dc.titlePrenylated flavonoid-enriched fraction from maclura tinctoria shows biological activity against Staphylococcus aureus and protects galleria mellonella larvae from bacterial infection.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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