Small scale screening of yeast strains enables high-throughput evaluation of performance in lignocellulose hydrolysates.

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dc.contributor.authorDijk, Marlous van
dc.contributor.authorTrollmann, Ignis
dc.contributor.authorSaraiva, Margarete Alice Fontes
dc.contributor.authorBrandão, Rogélio Lopes
dc.contributor.authorOlsson, Lisbeth
dc.contributor.authorNygård, Yvonne
dc.date.accessioned2021-12-07T20:50:46Z
dc.date.available2021-12-07T20:50:46Z
dc.date.issued2020pt_BR
dc.description.abstractSecond generation biorefineries demand efficient lignocellulosic hydrolysate fermenting strains and recent advances in strain isolation and engineering have progressed the bottleneck in developing production hosts from generation of strains into testing these under relevant conditions. In this paper, we introduce a methodology for high-throughput analysis of yeast strains directly in lignocellulosic hydrolysates. The Biolector platform was used to assess aerobic and anaerobic growth of 12 Saccharomyces cerevisiae strains and their ΔPdr12 mutants in wheat straw hydrolysate. The strains evaluated included lab, industrial and wild type strains and the screening could capture significant differences in growth and ethanol production among the strains. The methodology was also demonstrated with corn stover hydrolysate and the results were in line with shake flask cultures. Our study demonstrates that growth in lignocellulosic hydrolysates could be rapidly monitored using 1 ml cultures and that measuring growth and product formation under relevant conditions are crucial for evaluating strain performance.pt_BR
dc.identifier.citationDIJK, M. V. et al. Small scale screening of yeast strains enables high-throughput evaluation of performance in lignocellulose hydrolysates. Bioresource Technology Reports, v. 11, p. 100532, 2020. Disponível em: <https://www.sciencedirect.com/science/article/pii/S2589014X20301535>. Acesso em: 10 jun. 2021.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.biteb.2020.100532pt_BR
dc.identifier.issn2589-014X
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/14114
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/). Fonte: o PDF do artigo.pt_BR
dc.subjectSaccharomyces cerevisiaept_BR
dc.subjectWheat straw hydrolysatept_BR
dc.subjectAdaptationpt_BR
dc.subjectBioethanolpt_BR
dc.titleSmall scale screening of yeast strains enables high-throughput evaluation of performance in lignocellulose hydrolysates.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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