An antigenic domain within a catalytically active leishmania infantum nucleoside triphosphate diphosphohydrolase (NTPDase 1) is a target of inhibitory antibodies.

Maia, Ana Carolina Ribeiro Gomes; Porcino, Gabriane Nascimento; Detoni, Michelle de Lima; Emídio, Nayara Braga; Marconato, Danielle Gomes; Pinto, Priscila de Faria; Fessel, Melissa Regina; Reis, Alexandre Barbosa; Juliano Neto, Luiz; Juliano, Maria Aparecida; Marques, Marcos José; Vasconcelos, Eveline Gomes

An antigenic domain within a catalytically active leishmania infantum nucleoside triphosphate diphosphohydrolase (NTPDase 1) is a target of inhibitory antibodies.

Date

2013

Authors

Maia, Ana Carolina Ribeiro Gomes

Porcino, Gabriane Nascimento

Detoni, Michelle de Lima

Emídio, Nayara Braga

Marconato, Danielle Gomes

Pinto, Priscila de Faria

Fessel, Melissa Regina

Reis, Alexandre Barbosa

Juliano Neto, Luiz

Juliano, Maria Aparecida

Abstract

Weidentified a shared B domainwithin nucleoside triphosphate diphosphohydrolases (NTPDases) of plants and parasites. Now, an NTPDase activity not affected by inhibitors of adenylate kinase and ATPases was detected in Leishmania infantum promastigotes. By non-denaturing gel electrophoresis of detergent-homogenized promastigote preparation, an active band hydrolyzing nucleosides di- and triphosphate was visualized and, following SDS-PAGE and silver staining was identified as a single polypeptide of 50 kDa. By Western blots, it was recognized by immune sera raised against potato apyrase (SA), r-pot B domain (SB), a recombinant polypeptide derived fromthe potato apyrase, and LbB1LJ (SC) or LbB2LJ (SD), synthetic peptides derived fromthe Leishmania NTPDase 1, and by serum samples from dogs with visceral leishmaniasis, identifying the antigenic L. infantum NTPDase 1 and, also, its conserved B domain (r83–122). By immunoprecipitation assays andWestern blots, immune sera SA and SB identified the catalytically active NTPDase 1 in promastigote preparation. In addition, the immune sera SB (44%) and SC or SD (87–99%) inhibited its activity, suggesting a direct effect on the B domain. By ELISA, 37%, 45% or 50% of 38 infected dogs were seropositive for r-pot B domain, LbB1LJ and LbB2LJ, respectively, confirming the B domain antigenicity.

Keywords

Potato apyrase, Conserved domain, Visceral leishmaniasis

Citation

MAIA, A. C. R. G. et al. An antigenic domain within a catalytically active leishmania infantum nucleoside triphosphate diphosphohydrolase (NTPDase 1) is a target of inhibitory antibodies. Parasitology International, v. 18, p.44-52, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1383576912001274>. Acesso em: 10 out. 2016.