Comparative evaluation of phenol and thimerosal as preservatives for a candidate vaccine against American cutaneous leishmaniasis.

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dc.contributor.authorMayrink, Wilson
dc.contributor.authorTavares, Carlos Alberto Pereira
dc.contributor.authorDeus, Rosângela Barbosa de
dc.contributor.authorPinheiro, Melina Barros
dc.contributor.authorGuimarães, Tânia Mara Pinto Dabés
dc.contributor.authorAndrade, Hélida Monteiro de
dc.contributor.authorCosta, Carlos Alberto da
dc.contributor.authorToledo, Vicente de Paulo Coelho Peixoto de
dc.date.accessioned2014-11-14T18:09:01Z
dc.date.available2014-11-14T18:09:01Z
dc.date.issued2010
dc.description.abstractFor decades thimerosal has been used as a preservative in the candidate vaccine for cutaneous leishmaniasis, which was developed by Mayrink et al. The use of thimerosal in humans has been banned due to its mercury content. This study addresses the standardization of phenol as a new candidate vaccine preservative. We have found that the proteolytic activity was abolished when the test was conducted using the candidate vaccine added to merthiolate (MtVac) as well as to phenol (PhVac). The Montenegro’s skin test conversion rates induced by MtVac and by PhVac was 68.06% and 85.9%, respectively, and these values were statistically significant (p < 0.05). The proliferative response of peripheral mononuclear blood cells shows that the stimulation index of mice immunized with both candidate vaccines was higher than the one in control animals (p < 0.05). The ability of the candidate vaccines to induce protection in C57BL/10 mice against a challenge with infective Leishmania amazonensis promastigotes was tested and the mice immunized with PhVac developed smaller lesions than the mice immunized with MtVac. Electrophoresis of phenol-preserved antigen revealed a number of proteins, which were better preserved in PhVac. These results do in fact encourage the use of phenol for preserving the immunogenic and biochemical properties of the candidate vaccine for cutaneous leishmaniasis.pt_BR
dc.identifier.citationMAYRINK, W. et al. Comparative evaluation of phenol and thimerosal as preservatives for a candidate vaccine against American cutaneous leishmaniasis. Memórias do Instituto Oswaldo Cruz, v. 105, p. 86-91, 2010. Disponível em: <http://www.scielo.br/pdf/mioc/v105n1/13.pdf>. Acesso em: 20 ago. 2014.pt_BR
dc.identifier.doihttp://dx.doi.org/10.1590/S0074-02762010000100013
dc.identifier.issn0074-0276
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/3854
dc.language.isoen_USpt_BR
dc.rights.licenseO periódico Memórias do Instituto Oswaldo Cruz permite que o Repositório Institucional da Universidade Federal de Ouro Preto (UFOP) deposite uma cópia eletrônica dos artigos publicados por esse periódico  em que ao menos um dos autores faça parte da comunidade cientifica da UFOP. Fonte: Licença concedida mediante prenchimento de formulário enviado no dia 12 dez. 2013.pt_BR
dc.subjectLeishmaniasispt_BR
dc.subjectCandidate vaccinept_BR
dc.subjectPhenolpt_BR
dc.subjectMerthiolatept_BR
dc.titleComparative evaluation of phenol and thimerosal as preservatives for a candidate vaccine against American cutaneous leishmaniasis.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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