Adhesion on yeast cell surface as a trapping mechanism of pathogenic bacteria by Saccharomyces probiotics.

Tiago, Fabiana da Conceição Pereira; Martins, Flaviano dos Santos; Souza, Éricka Lorenna de Sales e; Pimenta, Paulo Filemon Paolucci; Araújo, Helena Rocha Corrêa de; Castro, Ieso de Miranda; Brandão, Rogélio Lopes; Nicoli, Jacques Robert

Adhesion on yeast cell surface as a trapping mechanism of pathogenic bacteria by Saccharomyces probiotics.

Date

2012

Authors

Tiago, Fabiana da Conceição Pereira

Martins, Flaviano dos Santos

Souza, Éricka Lorenna de Sales e

Pimenta, Paulo Filemon Paolucci

Araújo, Helena Rocha Corrêa de

Castro, Ieso de Miranda

Brandão, Rogélio Lopes

Nicoli, Jacques Robert

Abstract

Recently, much attention has been given to the use of probiotics as an adjuvant for the prevention or treatment of gastrointestinal pathology. The great advantage of therapy with probiotics is that they have few side effects such as selection of resistant bacteria or disturbance of the intestinal microbiota, which occur when antibiotics are used. Adhesion of pathogenic bacteria onto the surface of probiotics instead of onto intestinal receptors could explain part of the probiotic effect. Thus, this study evaluated the adhesion of pathogenic bacteria onto the cell wall of Saccharomyces boulardii and Saccharomyces cerevisiae strains UFMG 905, W303 and BY4741. To understand the mechanism of adhesion of pathogens to yeast, cell-wall mutants of the parental strain of Saccharomyces cerevisiae BY4741 were used because of the difficulty of mutating polyploid yeast, as is the case for Saccharomyces cerevisiae and Saccharomyces boulardii. The tests of adhesion showed that, among 11 enteropathogenic bacteria tested, only Escherichia coli, Salmonella Typhimurium and Salmonella Typhi adhered to the surface of Saccharomyces boulardii, Saccharomyces cerevisiae UFMG 905 and Saccharomyces cerevisiae BY4741. The presence of mannose, and to some extent bile salts, inhibited this adhesion, which was not dependent on yeast viability. Among 44 cell-wall mutants of Saccharomyces cerevisiae BY4741, five lost the ability to fix the bacteria. Electron microscopy showed that the phenomenon of yeast–bacteria adhesion occurred both in vitro and in vivo (in the digestive tract of dixenic mice). In conclusion, some pathogenic bacteria were captured on the surface of Saccharomyces boulardii, Saccharomyces cerevisiae UFMG 905 and Saccharomyces cerevisiae BY4741, thus preventing their adhesion to specific receptors on the intestinal epithelium and their subsequent invasion of the host.

Citation

TIAGO, F. da C. P. et al. Adhesion on yeast cell surface as a trapping mechanism of pathogenic bacteria by Saccharomyces probiotics. Journal of Medical Microbiology, v. 61, p. 1194-1207, 2012. Disponível em: <http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.042283-0#tab2> . Acesso em: 16 jun. 2017.