Intramuscular immunization with p36(LACK) DNA vaccine induces IFN-gama production but does not protect BALB/c mice against Leishmania chagasi intravenous challenge.

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dc.contributor.authorSilva, Eduardo de Almeida Marques da
dc.contributor.authorCoelho, Eduardo Antônio Ferraz
dc.contributor.authorGomes, Daniel Cláudio de Oliveira
dc.contributor.authorVilela, Márcia de Carvalho
dc.contributor.authorMasioli, Cássio Zumerle
dc.contributor.authorTavares, Carlos Alberto Pereira
dc.contributor.authorFernandes, Ana Paula
dc.contributor.authorAfonso, Luís Carlos Crocco
dc.contributor.authorRezende, Simone Aparecida
dc.date.accessioned2017-04-07T14:55:57Z
dc.date.available2017-04-07T14:55:57Z
dc.date.issued2005
dc.description.abstractAcute visceral leishmaniasis is a progressive disease caused by Leishmania chagasi in South America. The acquisition of immunity following infection suggests that vaccination is a feasible approach to protect against this disease. Since Leishmania homologue of receptors for activated C kinase (LACK) antigen is of particular interest as a vaccine candidate because of the prominent role it plays in the pathogenesis of experimental Leishmania major infection, we evaluated the potential of a p36(LACK) DNA vaccine in protecting BALB/c mice challenged with L. chagasi. In this study, mice received intramuscular (i.m.) or subcutaneous (s.c.) doses of LACK DNA vaccine. We evaluated the production of vaccine-induced cytokines and whether this immunization was able to reduce parasite load in liver and spleen. We detected a significant production of interferon gamma by splenocytes from i.m. vaccinated mice in response to L. chagasi antigen and to rLACK protein. However, we did not observe a reduction in parasite load neither in liver nor in the spleen of vaccinated animals. The lack of protection observed may be explained by a significant production of IL-10 induced by the vaccine.pt_BR
dc.identifier.citationSILVA, E. A. M. da et al. Intramuscular immunization with p36(LACK) DNA vaccine induces IFN-gama production but does not protect BALB/c mice against Leishmania chagasi intravenous challenge. Parasitology Research, v. 98, n.1, p. 67-74, 2005. Disponível em: <http://link.springer.com/article/10.1007%2Fs00436-005-0008-8>. Acesso em: 20 jan. 2017.pt_BR
dc.identifier.doihttps://doi.org/10.1007/s00436-005-0008-8
dc.identifier.issn1432-1955
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/7577
dc.identifier.uri2http://link.springer.com/article/10.1007%2Fs00436-005-0008-8pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectLackpt_BR
dc.titleIntramuscular immunization with p36(LACK) DNA vaccine induces IFN-gama production but does not protect BALB/c mice against Leishmania chagasi intravenous challenge.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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