The ubiquitin proteasome system in Strongyloididae. Biochemical evidence for developmentally regulated proteolysis in Strongyloides venezuelensis.

Paula, Fabiana Martins de; Borges, William de Castro; Pereira Junior, Olavo dos Santos; Gomes, Matheus de Souza; Ueta, Marlene Tiduko; Rodrigues, Vanderlei

The ubiquitin proteasome system in Strongyloididae. Biochemical evidence for developmentally regulated proteolysis in Strongyloides venezuelensis.

Date

2009

Authors

Paula, Fabiana Martins de

Borges, William de Castro

Pereira Junior, Olavo dos Santos

Gomes, Matheus de Souza

Ueta, Marlene Tiduko

Rodrigues, Vanderlei

Abstract

Nematode parasites from the genus Strongyloides spp. are important pathogens of the intestinal mucosa of animals and humans. Their complex life cycles involve alternating developmental adaptations between larvae stages and the adult parthenogenetic female. Here, we report, primarily through homology-based searching, the existence of the major components of the ubiquitin– proteasome system in this genus, using the available EST data from S. ratti, S. stercoralis, and Parastrongyloides trichosuri. In this study, S. venezuelensis was used as our model organism for detection of proteasome activity and ubiquitinated substrates in cytosolic preparations from the L3 larvae and the adult female. Marked differences in proteasome capabilities were found when these two stages were compared. A preference for degradation of chymotryptic synthetic peptides was found in both stages with the adult exhibiting a higher rate of hydrolysis compared to the larvae. Due to the high evolutionary conservation of proteasome alpha subunits, an anti-human proteasome antibody was able to recognize proteasome subunits in these preparations by Western blotting, supporting the proposal that the activity of the ubiqutin–proteasome system is developmentally regulated in this nematode.

Citation

PAULA, F. M. de et al. The ubiquitin proteasome system in Strongyloididae. Biochemical evidence for developmentally regulated proteolysis in Strongyloides venezuelensis. Parasitology Research, v. 105, p. 567-576, 2009. Disponível em: <https://link.springer.com/article/10.1007%2Fs00436-009-1430-0>. Acesso em: 20 mar. 2017.