The ubiquitin proteasome system in Strongyloididae. Biochemical evidence for developmentally regulated proteolysis in Strongyloides venezuelensis.

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dc.contributor.authorPaula, Fabiana Martins de
dc.contributor.authorBorges, William de Castro
dc.contributor.authorPereira Junior, Olavo dos Santos
dc.contributor.authorGomes, Matheus de Souza
dc.contributor.authorUeta, Marlene Tiduko
dc.contributor.authorRodrigues, Vanderlei
dc.date.accessioned2017-07-03T17:56:24Z
dc.date.available2017-07-03T17:56:24Z
dc.date.issued2009
dc.description.abstractNematode parasites from the genus Strongyloides spp. are important pathogens of the intestinal mucosa of animals and humans. Their complex life cycles involve alternating developmental adaptations between larvae stages and the adult parthenogenetic female. Here, we report, primarily through homology-based searching, the existence of the major components of the ubiquitin– proteasome system in this genus, using the available EST data from S. ratti, S. stercoralis, and Parastrongyloides trichosuri. In this study, S. venezuelensis was used as our model organism for detection of proteasome activity and ubiquitinated substrates in cytosolic preparations from the L3 larvae and the adult female. Marked differences in proteasome capabilities were found when these two stages were compared. A preference for degradation of chymotryptic synthetic peptides was found in both stages with the adult exhibiting a higher rate of hydrolysis compared to the larvae. Due to the high evolutionary conservation of proteasome alpha subunits, an anti-human proteasome antibody was able to recognize proteasome subunits in these preparations by Western blotting, supporting the proposal that the activity of the ubiqutin–proteasome system is developmentally regulated in this nematode.pt_BR
dc.identifier.citationPAULA, F. M. de et al. The ubiquitin proteasome system in Strongyloididae. Biochemical evidence for developmentally regulated proteolysis in Strongyloides venezuelensis. Parasitology Research, v. 105, p. 567-576, 2009. Disponível em: <https://link.springer.com/article/10.1007%2Fs00436-009-1430-0>. Acesso em: 20 mar. 2017.pt_BR
dc.identifier.doihttps://doi.org/10.1007/s00436-009-1430-0
dc.identifier.issn1432-1955
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/8137
dc.identifier.uri2https://link.springer.com/article/10.1007%2Fs00436-009-1430-0pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.titleThe ubiquitin proteasome system in Strongyloididae. Biochemical evidence for developmentally regulated proteolysis in Strongyloides venezuelensis.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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