Labeling PLA-PEG nanocarriers with IR780 : physical entrapment versus covalent attachment to polylactide.

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dc.contributor.authorMachado, Marina Guimarães Carvalho
dc.contributor.authorLana, Gwenaelle Elza Nathalie Pound
dc.contributor.authorOliveira, Maria Alice de
dc.contributor.authorLanna, Elisa Gomes
dc.contributor.authorFialho, Márcia Célia Pacheco
dc.contributor.authorBrito, Ana Carolina Ferreira de
dc.contributor.authorBarboza, Ana Paula Moreira
dc.contributor.authorSoares, Rodrigo Dian de Oliveira Aguiar
dc.contributor.authorMosqueira, Vanessa Carla Furtado
dc.date.accessioned2021-12-07T21:20:07Z
dc.date.available2021-12-07T21:20:07Z
dc.date.issued2020pt_BR
dc.description.abstractNear-infrared fluorescent dyes, such as IR780, are promising theranostics, acting as photosensitizers for photodynamic therapy and in vivo tracers in image-guided diagnosis. This work compared the uptake by macrophage-like cells of IR780 either physically associated or covalently attached to poly(D,L-lactide) (PLA) formulated as polymeric nanocapsules (NC) from a blend of PLA homopolymer and PLA-PEG block copolymer. The physicochemical characterization of both NC was conducted using asymmetric flow field-flow fractionation (AF4) analysis with static and dynamic light scattering and atomic force micros copy. The interaction of IR780 with serum proteins was evidenced by AF4 with fluorescence detection and flow cytometry in cell uptake studies. The average diameters of NC were around 120 nm and zeta potentials close to -40 mV for all NC. NC uptake by cells in different media and experimental conditions shows significantly lower fluorescence intensities for IR780 covalently linked to PLA and correspondingly low quantitative uptake. Different mechanisms of internalization were evidenced depending on the IR780 type of association to NC. Serum proteins mediate IR780 interaction with cells in a dose-dependent manner. Our results show that non-covalently linked IR780 was released from NC and accumulated in macrophage cells. Oppositely, IR780 conjugated to PLA provides stable association with NC, and its fluorescence is representative of cell uptake of the nanocarrier itself. This work strongly reinforces the importance of covalent attachment of a fluorescence dye such as IR780 to the nanocarrier to study their interaction with cells in vitro and to obtain reliable tracking in image-guided therapy.pt_BR
dc.identifier.citationMACHADO, M. G. C. et al. Labeling PLA-PEG nanocarriers with IR780: physical entrapment versus covalent attachment to polylactide. Drug Delivery and Translational Research, v. 10, p. s13346-020-0081, 2020. Disponível em: <https://link.springer.com/article/10.1007/s13346-020-00812-6>. Acesso em: 10 jun. 2021.pt_BR
dc.identifier.doihttps://doi.org/10.1007/s13346-020-00812-6pt_BR
dc.identifier.issn1520-5762
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/14118
dc.identifier.uri2https://link.springer.com/article/10.1007/s13346-020-00812-6pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectNanocapsulespt_BR
dc.subjectPolymer conjugationpt_BR
dc.subjectTheranosticspt_BR
dc.subjectCell uptakept_BR
dc.subjectMacrophagept_BR
dc.titleLabeling PLA-PEG nanocarriers with IR780 : physical entrapment versus covalent attachment to polylactide.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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