Characterization of two isolates of Trypanosoma cruzi obtained from the patient Berenice, the first human case of Chagas disease.

Lana, Marta de; Chiari, Cléa de Andrade; Chiari, Egler; Morel, Carlos Medicis; Gonçalves, Antônio M.; Romanha, Alvaro José

Characterization of two isolates of Trypanosoma cruzi obtained from the patient Berenice, the first human case of Chagas disease.

Date

1996

Authors

Lana, Marta de

Chiari, Cléa de Andrade

Chiari, Egler

Morel, Carlos Medicis

Gonçalves, Antônio M.

Romanha, Alvaro José

Abstract

Two isolates of Trypanosoma cruzi were obtained from the patient Berenice, the first human case of Chagas’ disease (Chagas 1909), when she was 55 and 71 years old, respectively. The isolates were characterized on the basis of their epimastigote-trypomastigote differentiation in liquid media and of the electrophoretic pattern of EcoR1 digestion products of kinetoplast DNA (k- DNA) minicircles (schizodeme) and isoenzyme patterns (zymodeme). Clear differences were found between the isolates, suggesting the occurrence of a heterogeneous population of T. cruzi in the infection of this patient.

Citation

LANA, M. de et al. Characterization of two isolates of Trypanosoma cruzi obtained from the patient Berenice, the first human case of Chagas disease. Parasitology Research, v. 82, p. 257-260, 1996. Disponível em: <http://link.springer.com/article/10.1007/s004360050106>. Acesso em: 20 jan. 2017.