Antioxidant study indicative of antibacterial and antimutagenic activities of an ellagitannin-rich aqueous extract from the leaves of Miconia latecrenata.

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dc.contributor.authorGontijo, Douglas da Costa
dc.contributor.authorGontijo, Pablo Costa
dc.contributor.authorBrandão, Geraldo Célio
dc.contributor.authorDiaz, Marisa Alves Nogueira
dc.contributor.authorOliveira, Alaíde Braga de
dc.contributor.authorFietto, Luciano Gomes
dc.contributor.authorLeite, João Paulo Viana
dc.date.accessioned2020-05-18T16:36:00Z
dc.date.available2020-05-18T16:36:00Z
dc.date.issued2019
dc.description.abstractEthnopharmacological relevance: Several plant species of Miconia genus are commonly used in Brazilian folk medicine as anti-inflammatory agents and for the treatment of infectious diseases. Infusions and extracts of Miconia species are also reported as analgesic, antimicrobial, antimalarial, antioxidant, anti-inflammatory, antinociceptive, antimutagenic, and antitumoral. Aim of the study; To determine the phytochemical composition of an aqueous extract of Miconia latecrenata leaves and to evaluate its antioxidant, antibacterial, antimutagenic and antigenotoxic activities. Materials and Methods: The following methods were used for the different effects: I) antioxidant - β-carotene/linoleic acid, lipid peroxidation, and DPPH• radical scavenging; II) antibacterial - agar well diffusion and MIC methods); III) antimutagenic assays - Ames Test; and IV) antigenotoxic - Plasmid cleavage test. The phytochemical analysis and phenolic quantification were carried out by UPLC-DAD-ESI-MS/MS and colorimetry, respectively. In addition, statistical correlation analysis was performed aiming to evaluate the Pearson correlation between phenolic compounds and biological assays. Results: A high content of tannins was observed and the ellagitannin isomers of 1,2,3,5-tris-galloyl-4,6-HHDP-glucose were identified as the main constituents of the leaves aqueous extract. High antioxidant effect, in different tests, high antibacterial activity to gram-positive and negative strains, as well as high antimutagenic activity were observed. Statistical analysis showed a high Pearson correlation for the tannin content in relation to the results of the antioxidant and antibacterial tests. In general, the antioxidant action of the aqueous extract showed low correlation with the antimutagenic activity. Conclusions: The present results confirmed the expectations regarding the pharmacological profile of M. latecrenata supporting its therapeutic potential in relation to ROS/RNS related disorders. Furthermore, the phenolic compounds of M. latecrenata can act, in turn, minimizing or inhibiting the biological macromolecules damage, especially DNA.pt_BR
dc.identifier.citationGONTIJO, D. C. et al. Antioxidant study indicative of antibacterial and antimutagenic activities of an ellagitannin-rich aqueous extract from the leaves of Miconia latecrenata. Journal of Ethnopharmacology, v. 236, p. 114-123, maio 2019. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0378874118348578?via%3Dihub>. Acesso em: 10 fev. 2020.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.jep.2019.03.007pt_BR
dc.identifier.issn0378-8741
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/12213
dc.identifier.uri2https://www.sciencedirect.com/science/article/pii/S0378874118348578?via%3Dihubpt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectMelastomataceaept_BR
dc.subjectROS - reactive oxygen speciespt_BR
dc.subjectRNS - neactive nitrogen speciespt_BR
dc.subjectPhenolic compoundspt_BR
dc.subjectLC-MS - liquid chromatography-mass spectrometrypt_BR
dc.titleAntioxidant study indicative of antibacterial and antimutagenic activities of an ellagitannin-rich aqueous extract from the leaves of Miconia latecrenata.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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