Capillary zone electrophoresis method to assay tipranavir capsules and identification of oxidation product and organic impurity by quadrupole-time of flight mass spectrometry.

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dc.contributor.authorLago, Matheus Wagner
dc.contributor.authorFriedrich, Mariane Lago
dc.contributor.authorIop, Gabrielle Dineck
dc.contributor.authorSouza, Thiago Belarmino de
dc.contributor.authorMello, Paola de Azevedo
dc.contributor.authorAdams, Andréa Inês Horn
dc.date.accessioned2019-04-22T12:12:41Z
dc.date.available2019-04-22T12:12:41Z
dc.date.issued2018
dc.description.abstractTipranavir (TPV) is one of the most recently developed protease inhibitors (PI) and it is specially recommended for treatment-experienced patients who are resistant to other PI drugs. In this work, a simple and friendly environmental CZE stability-indicating method to assay TPV capsules was developed and two TPV organic impurities were identified by high resolution mass spectrometry (HRMS). The optimized analytical conditions were: background electrolyte composed of sodium borate 50 mM, pH 9.0 and 5% of methanol; voltage + 28 kV; hydrodynamic injection of 5 s (100 mbar), detection wavelength 240 nm, at 25 °C. The separation was achieved in a fused silica capillary with 50 µm × 40 cm (inner diameter × effective length), using furosemide as internal standard. All the validation parameters were met and the method was specific, even in the presence of degradation products and impurities. Oxidation was indicated as the main degradation pathway among those evaluated in this study (acidic, alkaline, thermal, photolytic and oxidative) and it showed a second order degradation kinetic, under the conditions used in this study. The main oxidation product and an organic impurity detected in the standard were characterized by Q-TOF, and both of them correspond to oxidation products of TPV.pt_BR
dc.identifier.citationLAGO, M. W. et al. Capillary zone electrophoresis method to assay tipranavir capsules and identification of oxidation product and organic impurity by quadrupole-time of flight mass spectrometry. Talanta, v. 181, p. 182-189, maio 2018. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0039914018300183?via%3Dihub>. Acesso em: 7 mar. 2019.pt_BR
dc.identifier.issn0039-9140
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/11079
dc.identifier.uri2https://www.sciencedirect.com/science/article/pii/S0039914018300183pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectStabilitypt_BR
dc.subjectDegradation productspt_BR
dc.subjectHigh resolution mass spectrometrypt_BR
dc.titleCapillary zone electrophoresis method to assay tipranavir capsules and identification of oxidation product and organic impurity by quadrupole-time of flight mass spectrometry.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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