Host-parasite interactions in chagas disease : genetically unidentical isolates of a single Trypanosoma cruzi strain identified In vitro via LSSP-PCR.
No Thumbnail Available
Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The present study aims at establishing whether the diversity in pathogenesis within a genetically diverse host population infected with a single polyclonal strain of Trypanosoma cruzi is due to selection of specific subpopulations within the strain. For this purpose we infected Swiss mice, a genetically diverse population, with the polyclonal strain of Trypanosoma cruzi Berenice-78 and characterized via LSSP-PCR the kinetoplast DNA of subpopulations isolated from blood samples collected from the animals at various times after inoculation (3, 6 and 12 months after inoculation). We examined the biological behavior of the isolates in acellular medium and in vitro profiles of infectivity in Vero cell medium. We compared the characteristics of the isolates with the inoculating strain and with another strain, Berenice 62, isolated from the same patient 16 years earlier. We found that one of the isolates had intermediate behavior in comparison with Berenice-78 and Berenice-62 and a significantly different genetic profile by LSSP-PCR in comparison with the inoculating strain. We hereby demonstrate that genetically distinct Trypanosoma cruzi isolates may be obtained upon experimental murine infection with a single polyclonal Trypanosoma cruzi strain.
Description
Keywords
Citation
PAIVA, N. C. N. de et al. Host-parasite interactions in chagas disease: genetically unidentical isolates of a single Trypanosoma cruzi strain identified In vitro via LSSP-PCR. Plos One, v. 10, p. 1-14, 2015. Disponível em: <http://journals.plos.org/plosone/article/asset?id=10.1371%2Fjournal.pone.0137788.PDF>. Acesso em: 16 jun. 2016.