Host-parasite interactions in chagas disease : genetically unidentical isolates of a single Trypanosoma cruzi strain identified In vitro via LSSP-PCR.

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dc.contributor.authorPaiva, Nívia Carolina Nogueira de
dc.contributor.authorVieira, Paula Melo de Abreu
dc.contributor.authorOliveri, Larissa Maris Rezende
dc.contributor.authorFonseca, Kátia da Silva
dc.contributor.authorLana, Gwenaelle Elza Nathalie Pound
dc.contributor.authorOliveira, Maykon Tavares de
dc.contributor.authorVeloso, Vanja Maria
dc.contributor.authorReis, Alexandre Barbosa
dc.contributor.authorTafuri, Washington Luiz
dc.contributor.authorCarneiro, Cláudia Martins
dc.date.accessioned2016-07-12T12:21:57Z
dc.date.available2016-07-12T12:21:57Z
dc.date.issued2015
dc.description.abstractThe present study aims at establishing whether the diversity in pathogenesis within a genetically diverse host population infected with a single polyclonal strain of Trypanosoma cruzi is due to selection of specific subpopulations within the strain. For this purpose we infected Swiss mice, a genetically diverse population, with the polyclonal strain of Trypanosoma cruzi Berenice-78 and characterized via LSSP-PCR the kinetoplast DNA of subpopulations isolated from blood samples collected from the animals at various times after inoculation (3, 6 and 12 months after inoculation). We examined the biological behavior of the isolates in acellular medium and in vitro profiles of infectivity in Vero cell medium. We compared the characteristics of the isolates with the inoculating strain and with another strain, Berenice 62, isolated from the same patient 16 years earlier. We found that one of the isolates had intermediate behavior in comparison with Berenice-78 and Berenice-62 and a significantly different genetic profile by LSSP-PCR in comparison with the inoculating strain. We hereby demonstrate that genetically distinct Trypanosoma cruzi isolates may be obtained upon experimental murine infection with a single polyclonal Trypanosoma cruzi strain.pt_BR
dc.identifier.citationPAIVA, N. C. N. de et al. Host-parasite interactions in chagas disease: genetically unidentical isolates of a single Trypanosoma cruzi strain identified In vitro via LSSP-PCR. Plos One, v. 10, p. 1-14, 2015. Disponível em: <http://journals.plos.org/plosone/article/asset?id=10.1371%2Fjournal.pone.0137788.PDF>. Acesso em: 16 jun. 2016.pt_BR
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0137788
dc.identifier.issn1932-6203
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/6571
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fonte: o próprio artigo.pt_BR
dc.titleHost-parasite interactions in chagas disease : genetically unidentical isolates of a single Trypanosoma cruzi strain identified In vitro via LSSP-PCR.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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