Phytochemical characterization and antioxidant, antibacterial and antimutagenic activities of aqueous extract from leaves of Alchornea glandulosa.
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Date
2018
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Abstract
Plant extracts exist as a complex matrix which serves as a source of numerous bioactive
metabolites. The ultra performance liquid chromatography with diode-array detection-coupled
electrospray ionization-mass spectrometry/mass spectrometry technique was used to characterize the aqueous extract from leaves of Alchornea glandulosa (EAG), a species popularly used to
treat gastrointestinal problems as an antiulcer agent. Quantification of phenolic derivatives was
determined using Folin–Ciocalteu and aluminum trichloride (AlCl3) methods. In addition, antioxidant (2,2-diphenyl-1-picrylhydrazyl [DPPH•
] radical scavenging, β-carotene–linoleic acid, and
lipid peroxidation), antibacterial (agar well diffusion method and minimum inhibitory concentration), antimutagenic (Ames test), and antigenotoxic (plasmid cleavage) assays were also
performed on this plant extract. The ellagitannin tris-galloyl-hexahydroxydiphenic acid-glucose
was identified as the predominant compound along with tannins as majority metabolites. EAG
showed high antioxidant activity accompanied by moderate antibacterial activity against
Staphylococcus aureus. The highest antimutagenic activity was observed for TA97 strain without
metabolic activation (S9) and with metabolic activation, TA100 and TA102 were completely
inhibited. In addition, EAG exhibited potential signs of antigenotoxic action. The high antioxidant and antimutagenic activity observed for EAG suggests important therapeutic uses that still
need to be verified in future studies.
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Keywords
Phenolic compounds, lchornea glandulosa, DNA damage, Antioxidant and Antibacterial Action
Citation
GONTIJO, D. da C. et al. Phytochemical characterization and antioxidant, antibacterial and antimutagenic activities of aqueous extract from leaves of Alchornea glandulosa. Journal of Toxicology and Environmental Health, Part A Current Issues, v. 81, n. 6, p. 2-14, 2018. Disponível em: <https://www.tandfonline.com/doi/abs/10.1080/15287394.2018.1492479?journalCode=uteh20>. Acesso em: 25 fev. 2019.