Browsing by Author "Ramos, Camila de Oliveira"

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    Associação entre medidas antropométricas, de composição corporal, bioquímicas e de consumo alimentar em indivíduos com excesso de peso.
    (2013) Ramos, Camila de Oliveira; Gomes, Arthur da Silva; Previato, Helena Dória Ribeiro de Andrade; Volp, Ana Carolina Pinheiro
    Avaliar a associação entre medidas antropométricas, de composição corporal, bioquímicas e de consumo alimentar em indivíduos com excesso de peso. Métodos: Estudo do tipo transversal, com amostra de conveniência composta por 31 indivíduos, com idade entre 19 e 59 anos. Foram realizadas avaliações antropométricas, bioquímicas e análise da composição da dieta. Após, foi calculado o índice de qualidade da dieta (IQD). Na análise estatística, o teste de Shapiro-Wilk verificou a distribuição das variáveis, Wilcoxon-Mann- Whitney-U as diferenças entre as variáveis e correlação de Spearman/Pearson rastreou a associação entre os valores. Foi adotado um p com 5% de probabilidade. Resultados: A análise da composição da dieta permitiu observar uma média de consumo acima das Dietary Reference Intakes para carboidrato (323,31±33,11g), proteína (80,15±29,84g), lipídeo (77,42±31,87g) e sódio (2896,99±1119,05mg). Os valores para o IQD variaram entre 2 e 12 pontos, apresentando valor médio de 6,5±2,2. Foram encontradas correlações entre IQD e prega cutânea suprailíaca, colesterol total, e LDL-c (mg/dL), dentre outras. Conclusão: A amostra estudada apresentou um perfil de ingestão alimentar composto por alimentos ricos em carboidratos, lipídeos e sódio. A elevada média da pontuação do IQD (dieta pobre) reflete a importância de medidas de intervenção visando à melhoria do padrão alimentar desses indivíduos.
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    Efeitos da administração da taurina no desequilíbrio redox e na inflamação induzida pela exposição à fumaça de cigarro.
    (2017) Ramos, Camila de Oliveira; Bezerra, Frank Silva; Chianca Júnior, Deoclécio Alves; Silva, Marcelo Eustáquio; Bezerra, Frank Silva
    A fumaça de cigarro (FC) é considerada o maior fator de risco para o desenvolvimento de doença pulmonar obstrutiva crônica (DPOC). A FC apresenta uma mistura complexa de mais de 7000 compostos químicos. Entre esses, encontram-se um grande número de espécies reativas de oxigênio (EROs) que leva ao desencadeamento do estresse oxidativo. Estudos in vivo têm demonstrado os benefícios do uso da taurina, um β-aminoácido, em diminuir a inflamação e o estresse oxidativo no sistema respiratório. Esse estudo teve como objetivo avaliar os efeitos da taurina no estresse oxidativo e na resposta inflamatória pulmonar de camundongos expostos à FC. Foram utilizados 50 camundongos machos da linhagem C57BL/6 divididos nos grupos: Controle (CT), taurina (TAU), fumaça de cigarro (FC), fumaça + taurina (FC+TAU). Durante cinco dias, por meio da utilização de uma câmara de inalação de fumaça de cigarro, os grupos FC e FC+TAU foram expostos a 4 cigarros, três vezes ao dia totalizando 12 cigarros comerciais. Os grupos TAU e FC+TAU receberam 30 mg/Kg/dia de taurina via gavagem orogástrica doze horas antes da exposição. 24h após a última exposição à fumaça de cigarro, foi realizada a eutanásia dos animais via sobredose de anestésico para coleta do sangue, lavado broncoalveloar (LBA) e amostras dos tecidos pulmonares para análises bioquímicas e para o processamento histopatológico. A análise dos parâmetros hematológicos demonstrou um aumento dos níveis de eritrócito, hemoglobina e hematócrito em FC quando comparado ao CT e TAU, porém, em FC+TAU houve uma redução de eritrócito quando comparado ao FC. O nível de leucócitos totais no sangue foi menor em FC quando comparado ao CT e TAU e foi observado um aumento nos níveis em FC+TAU quando comparado ao FC. Observou-se um aumento de leucócitos totais no LBA em FC quando comparado ao CT e TAU, entretanto, em FC+TAU observou-se uma redução dos níveis de células inflamatórias quando comparado ao FC. A contagem diferencial de células inflamatórias no LBA demonstrou um aumento de macrófago em FC quando comparado ao CT e TAU e a administração de taurina associada à FC levou a uma redução dos níveis de macrófagos em comparação ao FC. Em relação ao ensaio imunoenzimático, observou-se um aumento na concentração do fator de necrose tumoral (TNF) e da interleucina (IL) -17 em FC e FC+TAU quando comparado ao CT e TAU, para a IL-22 houve um aumento em FC quando comparado ao CT e TAU, porém, em FC+TAU foi observado uma redução comparado ao FC. Na análise do dano oxidativo, observou-se aumento da peroxidação lipídica e da oxidação de proteína em FC quando comparado ao CT e TAU, esses danos foram reduzidos em FC+TAU quando comparado ao FC. Para a atividade das enzimas antioxidantes superóxido dismutase (SOD) e catalase (CAT) observou-se maior atividade em FC comparado ao CT a TAU e uma redução em FC+TAU comparado ao FC. Em relação aos dados morfométricos, observou-se aumento da densidade e volume do espaço alveolar (Vv[a]) em FC comparado ao CT e TAU e diminuição da densidade de volume de septo alveolar (Vv[sa]) comparado ao CT e TAU. A administração de taurina demonstrou ser capaz de reduzir a inflamação e o estresse oxidativo induzido pela exposição à fumaça de cigarro em um curto período de tempo.
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    EPA/DHA and linseed oil have different effects on liver and adipose tissue in rats fed with a high-fat diet.
    (2022) Dias, Bruna Vidal; Gomes, Sttefany Viana; Castro, Maria Laura da Cruz; Carvalho, Luana Cristina Faria; Breguez, Gustavo Silveira; Souza, Débora Maria Soares de; Ramos, Camila de Oliveira; Sant’Ana, Marcella Ramos; Nakandakari, Susana Castelo Branco Ramos; Araujo, Carolina Morais; Guimaraes, Andrea Grabe; Silva, André Talvani Pedrosa da; Carneiro, Cláudia Martins; Cintra, Dennys Esper Corrêa; Costa, Daniela Caldeira
    The incidence of cardiovascular diseases and metabolic disorders has increased worldwide. Clinical and exper- imental research has shown that the consumption of ω-3 FAs can be beneficial to metabolism in several ways, as they can act on metabolic pathways. Our objective was to evaluate the effect of treatment with linseed oil, a vegetable oil rich in alpha-linolenic acid, and EPA and DHA in different proportions (3:1 EPA:DHA, and 1:3 EPA: DHA), on the metabolic disorders induced by a high-fat diet (20 % lipids) in rats for 2 weeks, after 18 weeks of consumption of a high-fat diet. In 18 weeks, the high-fat diet increased blood glucose, systolic blood pressure, triglyceride concentration in the liver and adipose tissue, and impaired insulin sensibility without interfering in the weight of the animals. All treatments were effective in reducing the deposition of hepatic type III collagen, the proportion of ω-6/ω-3 in the liver and WAT (white adipose tissue), the proportion of area/number of adi- pocytes, and the gene expression of the ACC, FAS, and CPT1 enzymes. In addition, treatment with EPA and DHA reduced blood glucose, serum TNF-α concentration, amount of liver fat, degree of microsteatosis and type I collagen deposition in the liver, deposition of type I and III collagen in TA, gene expression of the transcription factor SREBP-1c, and increased hepatic binucleation. EPA in major proportion was more effective in reducing the area of adipocytes, hepatic triglyceride concentration, PPAR-α expression, and WAT fat weight. DHA in a major proportion reduced the concentration of MCP1 in WAT. LO treatment did not have any isolated effects. We concluded that EPA and DHA were more effective in treating metabolic damage than treatment with LO, leading to a more favorable metabolic profile.
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    Exogenous surfactant prevents hyperoxiainduced lung injury in adult mice.
    (2019) Bezerra, Frank Silva; Ramos, Camila de Oliveira; Castro, Thalles de Freitas; Araújo, Natália Pereira da Silva; Souza, Ana Beatriz Farias de; Bandeira, Ana Carla Balthar; Costa, Guilherme de Paula; Cartelle, Christiane Teixeira; Silva, André Talvani Pedrosa da; Cangussú, Silvia Dantas; Brochard, Laurent; Nagato, Akinori Cardozo
    Background: In addition to the risk of developing ventilator-induced lung injury, patients with ARDS are at risk of developing hyperoxic injury due the supra-physiological oxygen supplementation clinically required to reverse hypoxemia. Alterations of endogenous surfactant system participate in the pulmonary dysfunction observed in ARDS. Administration of exogenous surfactant could have protective effects during hyperoxia. Methods: Male BALB/c mice (8–10 weeks), a strain highly sensitive to hyperoxia, received the exogenous surfactant-containing protein SP-B and SP-C by intranasal instillation 12 h before starting 24 h of exposure to hyperoxia in an inhalation chamber and were compared to mice receiving hyperoxia alone and to controls subjected to normoxia. Results: Compared to the hyperoxia group, the administration of exogenous surfactante was able to reduce lung inflammation through a reduction in the influx of neutrophils and inflammatory biomarkers such as TNF, IL-17, and HMGB1 expression. The antioxidante activity prevented oxidative damage by reducing lipid peroxidation and protein carbonylation and increasing superoxide dismutase activity when compared to the hyperoxia group. Conclusion: Our results offer new perspectives on the effects and the mechanism of exogenous surfactant in protecting the airway and lungs, in oxygen-rich lung microenvironment, against oxidative damage and aggravation of acute inflammation induced by hyperoxia.
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    Lycopene mitigates pulmonary emphysema induced by cigarette smoke in a murine model.
    (2019) Campos, Keila Karine Duarte; Ramos, Camila de Oliveira; Martins, Thais Lourenço; Costa, Guilherme de Paula; Silva, André Talvani Pedrosa da; Garcia, Camila Carrião Machado; Oliveira, Laser Antônio Machado de; Cangussú, Silvia Dantas; Costa, Daniela Caldeira; Bezerra, Frank Silva
    Chronic obstructive pulmonary disease (COPD) is an inflammatory lung disease characterized by a non-fully reversible airflow limitation comprising chronic bronchitis and pulmonary emphysema both being induced by cigarette smoke (CS) exposure. Lycopene has shown antioxidant and anti-inflammatory properties that can prevent acute lung inflammation and emphysema. We hypothesized that administration with lycopene would repair lung damage in emphysema caused by CS exposure. Mice were administered with two different doses of lycopene (25 or 50 mg/kg/day, diluted in sunflower oil by orogastric gavage) and then exposed to 60 days of CS or not (CG). Lycopene promoted a reduction in the number of total leukocytes and it improved pulmonary emphysema. Lycopene was able to minimize redox processes by decreasing lipid peroxidation and DNA damage, and by having an increase in the activities of SOD, CAT and GSH content. Furthermore, it decreased levels of TNF-α, IFN-γ and IL-10. In addition, it was able to decrease MPO activity and nitrite content. In conclusion, our data elucidated the role of lycopene as an antioxidant and anti-inflammatory agent in mice exposed to CS.
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    Taurine treatment decreases inflammation and oxidative stress in lungs of adult mice exposed to cigarette smoke.
    (2018) Ramos, Camila de Oliveira; Campos, Keila Karine Duarte; Costa, Guilherme de Paula; Cangussú, Silvia Dantas; Silva, André Talvani Pedrosa da; Bezerra, Frank Silva
    Taurine is the major free amino acid found in mammalian cells and is known to be an antioxidant and membrane-stabilizing agent. This study aimed to evaluate the effects of taurine on oxidative stress and inflammatory response in the lungs of mice exposed to cigarette smoke. Fifty male C57BL/6 mice were divided into 5 groups: control group (CG), vehicle group (VG), taurine group (TG), cigarette smoke group (CSG), and cigarette smoke + taurine group (CSTG). For five consecutive days, CSG and CSTG were exposed to 4 cigarettes 3 times a day. Taurine administration was able to reduce total leukocytes in bronchoalveolar lavage fluid in CSTG compared to CSG. There was an increase in antioxidant superoxide dismutase and catalase activity in CSG compared to that in CG and TG, and a decrease in CSTG compared to CSG. There was an increase in the concentration of TNF and IL-17 in CSG and CSTG compared to CG and TG. There was an increase in the concentration of IL-22 in CSG compared to CG and TG, and a decrease in CSTG compared to CSG. The administration of taurine has been shown to reduce the inflammation and oxidative stress induced by short-term exposure to cigarette smoke.
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    The administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke.
    (2018) Machado, Dafne Fernandes; Campos, Keila Karine Duarte; Silva, Natália Pereira da; Ramos, Camila de Oliveira; Cangussú, Silvia Dantas; Costa, Guilherme de Paula; Silva, André Talvani Pedrosa da; Bezerra, Frank Silva
    The alveolar surfactant, which composition consists of a unique and complex mixture of lipids and proteins, has immunomodulatory action. This study aimed to evaluate the effects of exogenous surfactant on pulmonary inflammatory response in mice exposed to cigarette smoke (CS). Twenty-four mice C57BL/6 were divided into four groups: control group exposed to ambient air (CG); surfactant treated group (SG); CS exposed group (CSG) and CS exposed group treated with surfactant (CSSG). For five days, CSG and CSSG were exposed to 12 commercial cigarettes/day and SG and CSSG received the surfactant by intranasal instillation. At the end of the experiment, the animals were euthanatized for the collection of bronchoalveolar lavage fluid (BALF) and lungs. The total number of leukocytes in BALF increased in CSG compared to CG, however, there was a decrease in CSSG compared to CSG. There was an increase in lipid peroxidation in SG and CSG compared to CG while there was a decrease in CSSG compared to CSG. Regarding the antioxidant enzymes, the catalase (CAT) activity increased in all groups compared to CG and the superoxide dismutase (SOD) activity decreased in CSG compared to the CG and SG. There was an increase in TNF in SG, CSG and CSSG compared to CG. There was an increase in IL-17 in CSSG compared to CG. There was an increase in CCL5 in SG and CSSG compared to CG. Therefore, our results demonstrated that the administration of exogenous surfactant was able to decrease the oxidative processes in the lungs of mice induced by short-term exposure to CS.
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    The exposure to formaldehyde causes renal dysfunction, inflammation and redox imbalance in rats.
    (2017) Ramos, Camila de Oliveira; Nardeli, Clarissa Rodrigues; Campos, Keila Karine Duarte; Pena, Karina Braga; Machado, Dafne Fernandes; Bandeira, Ana Carla Balthar; Costa, Guilherme de Paula; Silva, André Talvani Pedrosa da; Bezerra, Frank Silva
    Twenty-eight Fischer male rats were divided into four groups: control group (CG), exposed to the ambient air, and groups exposed to formaldehyde (FA) at concentrations of 1% (FA1%), 5% (FA5%) and 10% (FA10%). Kidney function was assessed by dosage of uric acid, creatinine and urea. Morphometry was performed on the thickness of the lumen of Bowman's capsule and diameter of the lumen of the renal tubules. We evaluated the redox imbalance through the catalase and superoxide dismutase activity as well as oxidative damage by lipid peroxidation. Inflammatory chemokines CCL2, CCL3 and CCL5 were analyzed by enzyme immunoassays. There was an increase in the concentration of urea in FA10% compared with CG and FA1%. The levels of creatinine, renal lumen and lipid peroxidation increased in all FA-treated groups compared with CG. The concentration of uric acid in FA10% was lower compared with all other groups. There was an increase in the space of Bowman's capsule in FA5% and FA10% compared with CG and FA1%. However, the superoxide dismutase activity was higher in FA5% compared with other groups while CCL5 was higher in FA1% compared with CG. The exposure to formaldehyde in a short period of time leads to changes in the kidney function, inflammation and morphology, as well as promoted the increase of superoxide dismutase activity and oxidative damage.
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    The β-blocker carvedilol and the benznidazole modulate the cardiac immune response in the acute infection induced by Colombian strain of the Trypanosoma cruzi.
    (2018) Horta, Aline Luciano; Figueiredo, Vivian Paulino; Leite, Ana Luísa Junqueira; Costa, Guilherme de Paula; Menezes, Ana Paula de Jesus; Ramos, Camila de Oliveira; Pedrosa, Tamiles Caroline Fernandes; Bezerra, Frank Silva; Vieira, Paula Melo de Abreu; Silva, André Talvani Pedrosa da
    BACKGROUND The infection led by Trypanosoma cruzi persists in mammalian tissues causing an inflammatory imbalance. Carvedilol (Cv), a non-selective beta blocker drug indicated to treat heart failure and antihypertensive has shown to promote antioxidant and immunomodulatory properties which might improve the inflammation induced by T. cruzi. OBJECTIVES Evaluate the role of Cv on the inflammatory response of C57BL/6 mice acutely infected with the Colombian strain of T. cruzi. METHODS Animals were infected with the Colombian strain of T. cruzi and treated with Cv (25 mg/kg/day), benznidazole (Bz) (100 mg/kg/day) or their combination. On the 28th day of infection and 23 days of treatment, the euthanasia occurred, and the heart preserved for histopathological, oxidative stress (SOD, catalase, TBARs, carbonylated proteins) and plasma (CCL2, CCL5, TNF, IL-10) analyses. Parasitaemia and survival were assessed along the infection. FINDINGS Cv decreased TBARs, but increased the mortality rate, the parasitaemia and the levels of CCL2, CCL5, catalase and the inflammatory infiltrate in the cardiac tissue. Bz led the reduction of the inflammatory infiltrate and circulating levels of oxidative stress and inflammatory mediators in the infected mice. MAIN CONCLUSIONS Our data suggest that Cv, in this experimental model using the Colombian strain of T. cruzi, caused damage to the host.